@misc{oai:repo.qst.go.jp:00067456,
 author = {Yamada, Yutaka and Oghiso, Yoichi and Nakamura, Shingo and 山田 裕 and 小木曽 洋一 and 中村 慎吾},
 month = {Aug},
 note = {Lifespan bone carcinogenesis was compared among C3H/He, C57BL/6 and B6C3F1 strains of female mice following injection of soluble Pu-239 citrate to clarify the specifi city for pathogenesis and molecular mechanisms of bone tumors. Bone tumors, mostly osteogenic sarcomas, appeared early from 200 to 600 days after the injection, showing almost similar dose responses with the peak incidence of 50-63% at the skeletal dose of 2-3 Gy, in total 630 animals from all the strains. The primary sites of bone tumors from these strains of mice were predominantly distributed in 80-90% of the skeletal bones with well-developed trabecular bone surfaces and large vascular sinusoids. Most of bone tumors appeared to be osteosarcomas derived from trabecular osteoblasts, while only a small part was giant cell sarcomas from osteoclasts as well as a few of fi brosarcomas. As far as the other tumors including lymphomyeloid neoplasms and the other solid tumors were less frequently or scarcely found in Pu-injected mice, osteosarcomas are only the specifi c tumor commonly observed among mouse strains with different genetic background. To examine whether any oncogene alterations were related to such osteosarcomagenesis, PCR-SSCP and direct sequence analyses were performed on DNA extracted from 11 fresh and frozen bone tumor specimens. Only one case, however, showed C to A transversion in codon 245 of exon 7 of p53 alone, while the other 10 cases showed neither p53 mutations nor K-, H-, N-ras mutations. These results indicate specifi c induction of osteosarcomas in mice early following exposures of skeletal bones to alpha-particles despite no evidence for p53 and ras mutations but the other genetic alterations which might be related to ontogenic and developmental stages of osteosarcomas., 第１２回国際放射線研究会議(ICRR2003)},
 title = {Induction of osteosarcomas and related oncogene alterations in mice after injections of 239Pu citrate},
 year = {2003}
}