@misc{oai:repo.qst.go.jp:00067433,
 author = {Kanai, Reiko and Katsuyoshi, Fujikawa and Nishimura, Mayumi and Azuma, Hiroto and Shimada, Yoshiya and Yamauchi, Masatake and 内山 禮子 and 藤川 勝義 and 西村 まゆみ and 東 裕人 and 島田 義也 and 山内 正剛},
 month = {Aug},
 note = {Genetic heterogeneity and a low frequency of germline mutation at single-copy gene loci have limited the direct measurement of germline mutation in human populations. Two confl icting results have been reported for the effect of ionizing radiation on germline mutation in human populations. A study conducted on the fi rst-generation progeny of the survivors of the atomic bombs at Hiroshima and Nagasaki found no signifi cant increase in germline mutations. On the other hand, a signifi cant increase in germline mutation was reported among the human population in the Belarus area after the Chernobyl accident in 1986. We investigated the germline mutation at the molecular level using experimental mouse strains with different genetic backgrounds to assess the risk of ionizing radiation on human populations. The C3H male parents were exposed to X ray (0, 0.3, 1, and 3Gy) and mated with unexposed C57BL females after two weeks interval, so as to detect the germline mutation occurred at the spermatid stage. Genomic DNA samples were prepared from the both parents and F1s, and the genomic DNA sequences were compared between parents and offspring at the specifi c genomic gene loci, such as adenine phosphoribosyl transferase (aprt) gene and cytidine triphosphate synthetase (ctps) gene, using the automated DNA sequencer. Also hypervariable Pc-1 (Ms6-hm) minisatellite repeat locus was analyzed by using Southern blot hybridization technique. Our preliminary results indicated that the changes of the restriction DNA fragment length in offspring did not refl ect the occurrence of the mutation, such as point mutation, insertion, and deletion, in the genomic gene loci including the intervening sequence (intron)., 第１２回国際放射線研究会議},
 title = {Molecular analysis on germline mutation caused by low-dose irradiation},
 year = {2003}
}