{"created":"2023-05-15T14:49:11.320678+00:00","id":67371,"links":{},"metadata":{"_buckets":{"deposit":"48bd8642-70ab-408a-b4b7-d931a0d9e89c"},"_deposit":{"created_by":1,"id":"67371","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"67371"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00067371","sets":["10:28"]},"author_link":["662286","662284","662285","662287"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2003-04-25","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"【目的】DNA多型は個体識別に利用されるが，特にゲノム中に数百万ヵ所存在すると推定されるSNPs（1塩基多型）は有力な情報である．本研究では，大量に蓄積された公共遺伝子データベース中の転写配列情報を元に，新たなSNPs候補の推定と検出をおこなう支援ツールの開発を目指した．【方法】多数の独立個体材料から取得されたmRNA配列約10万および部分的mRNA配列約470万件を，塩基配列相同性によりグループ化し複数配列どうしの共通領域における矛盾塩基をSNP候補としてリスト化した．ここから他の多型データベースで指摘されていない候補について，日本人24人（48アレル）のゲノムDNAを鋳型に当該領域の塩基配列をPCR増幅し塩基配列を決定した．【成績】期待値が10-30％の候補は約1割，期待値30-40％の候補は約3割が確認された．【結論】従来指摘のないエクソン領域SNP候補が10万件以上見つかり，これらの一定数が日本人集団においても検出可能である．","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第87次日本法医学会総会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"斎藤, 俊行"}],"nameIdentifiers":[{"nameIdentifier":"662284","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"林, 昭子"}],"nameIdentifiers":[{"nameIdentifier":"662285","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"齋藤 俊行","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"662286","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"林 昭子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"662287","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"転写配列クラスタリングによる新規SNPsの同定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"転写配列クラスタリングによる新規SNPsの同定"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2003-04-30"},"publish_date":"2003-04-30","publish_status":"0","recid":"67371","relation_version_is_last":true,"title":["転写配列クラスタリングによる新規SNPsの同定"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:38:31.011533+00:00"}