{"created":"2023-05-15T14:48:55.050758+00:00","id":67009,"links":{},"metadata":{"_buckets":{"deposit":"aaca0a3e-08a3-4311-831c-db2a09224ec2"},"_deposit":{"created_by":1,"id":"67009","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"67009"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00067009","sets":["10:29"]},"author_link":["658712","658710","658713","658714","658711"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2018-12-01","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"【Purpose】D-Tagatose is a naturally occurring ketohexose considered to be a potential reduced-energy sweetener. For industrial application, we will modify L-arabinose isomerase (L-AI) from a thermophilic microorganism, Geobacillus stearothermophilus to improve its substrate specificity for D-galactose for the production of D-tagatose.\n【Method and Result】Analysis of residues around the binding pocket was conducted using molecular operating environment (MOE) software, and modification of L-AI was performed using saturation mutagenesis. Among the selected residues, mutation at residue 18 produced a mutant strain, H18T, which exhibited increased activity for D-galactose compared with the wild-type (WT) enzyme. H18T exhibited higher substrate binding and catalytic efficiency of about 2.7-fold and 1.8-fold, respectively, for D-galactose. H18T demonstrated improved substrate specificity for D-galactose by up to 45.4%.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第25回日本生物工学会 九州支部鹿児島大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Amreta, Laksmi Fina"}],"nameIdentifiers":[{"nameIdentifier":"658710","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Arai, Shigeki"}],"nameIdentifiers":[{"nameIdentifier":"658711","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsurumaru, Hirohito"}],"nameIdentifiers":[{"nameIdentifier":"658712","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishibashi, Matsujiro"}],"nameIdentifiers":[{"nameIdentifier":"658713","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"新井 栄揮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"658714","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Improved substrate specificity for D-galactose of L-arabinose isomerase for industrial application","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Improved substrate specificity for D-galactose of L-arabinose isomerase for industrial application"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-28"},"publish_date":"2018-11-28","publish_status":"0","recid":"67009","relation_version_is_last":true,"title":["Improved substrate specificity for D-galactose of L-arabinose isomerase for industrial application"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:42:32.274323+00:00"}