{"created":"2023-05-15T14:48:33.314659+00:00","id":66521,"links":{},"metadata":{"_buckets":{"deposit":"56721a7b-53fc-4695-9d1c-7a46e5409c79"},"_deposit":{"created_by":1,"id":"66521","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"66521"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00066521","sets":["10:29"]},"author_link":["654346","654348","654342","654351","654350","654353","654345","654341","654352","654344","654347","654349","654354","654355","654356","654343"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2017-10-25","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"【背景・目的】D-メチオニンは硫黄を含むアミノ酸の一種であり、重粒子線照射時にマウスの唾液腺に対して、明白な副作用なく、放射線防護効果を示す。本研究では、他に有望なアミノ酸がないか調べるため、まず重粒子線誘発DNA損傷に対するアミノ酸の保護効果を調べ、メチオニンと比較した。\n【方法】重粒子線によるDNA損傷を、 プラスミド DNAの形状変化からDNA電気泳動法によって定量化した。DNA溶液にアミノ酸を加え、重粒子線を照射した（放射線医学総合研究所HIMAC, 炭素イオン線 290MeV/u）。アミノ酸は、システイン(Cys)、 トリプトファン(Trp)、 メチオニン(Met)、 フェニルアラニン(Phe)、 バリン(Val)、 アラニン(Ala)をテストした。緩衝溶液は、トリスとリン酸緩衝溶液を用いた。\n【結果・考察】重粒子線誘発のSSBに対する保護効果は、Ala < Val < Met < Phe ~ Trp < Cys の順であった。DSBに対する保護効果は、Ala < Val < Met < Phe ~ Cys < Trpであった。SSBおよびDSBに対するG値とアミノ酸のOHラジカルの消去能力との間に相関が見られた。これらのデータは、重粒子線誘発のプラスミドDNA損傷に対するアミノ酸の保護効果が、OHラジカルの消去作用で説明できる可能性を示唆していた。\n【結論】メチオニンと同様に、トリプトファン、システイン、フェニルアラニンは、重粒子線誘発のプラスミドDNA損傷に対して保護効果を示した。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会第60回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"余語, 克紀"}],"nameIdentifiers":[{"nameIdentifier":"654341","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"村山, 千恵子"}],"nameIdentifiers":[{"nameIdentifier":"654342","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"前山, 拓哉"}],"nameIdentifiers":[{"nameIdentifier":"654343","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平山, 亮一"}],"nameIdentifiers":[{"nameIdentifier":"654344","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本, 謙一郎"}],"nameIdentifiers":[{"nameIdentifier":"654345","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石山, 博條"}],"nameIdentifiers":[{"nameIdentifier":"654346","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小澤, 修一"}],"nameIdentifiers":[{"nameIdentifier":"654347","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"早川, 和重"}],"nameIdentifiers":[{"nameIdentifier":"654348","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"永田, 靖"}],"nameIdentifiers":[{"nameIdentifier":"654349","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"余語 克紀","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"654350","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"村山 千恵子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"654351","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"前山 拓哉","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"654352","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平山 亮一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"654353","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本 謙一郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"654354","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"早川 和重","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"654355","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"永田 靖","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"654356","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"重粒子線によるDNA損傷に対するアミノ酸の保護効果","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"重粒子線によるDNA損傷に対するアミノ酸の保護効果"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-11-17"},"publish_date":"2017-11-17","publish_status":"0","recid":"66521","relation_version_is_last":true,"title":["重粒子線によるDNA損傷に対するアミノ酸の保護効果"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:48:01.810325+00:00"}