{"created":"2023-05-15T14:47:54.249482+00:00","id":65656,"links":{},"metadata":{"_buckets":{"deposit":"3afe9c40-0471-4cac-92b3-654688cfc932"},"_deposit":{"created_by":1,"id":"65656","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"65656"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00065656","sets":["10:29"]},"author_link":["646738","646739","646745","646746","646736","646743","646737","646747","646749","646742","646750","646752","646748","646741","646751","646744","646740"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2015-06-11","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"【目的】抗酸化物質の活性評価に用いるDPPHラジカルは水に溶けないため、水溶液中で用いる場合にはエタノールなどの共溶媒が必要である。このためpHを制御する際には緩衝塩が析出するという問題があった。我々は最近、DPPHラジカルをβ-シクロデキストリン(β-CD)に包接させることで水溶化することに成功した(Chem Commn 2015; 51: 8311-8314)。本研究では、この水溶化DPPHラジカルを用い、水溶性抗酸化物質の速度論的活性評価を行った。\n【方法】DPPHラジカルとβ-CDに沸騰水を加えて攪拌し、室温まで冷却後、孔径0.22 μmのメンブレンフィルターでろ過することによりDPPHラジカル水溶液を得た。反応の追跡にはユニソクRSP-1000-02型ストップトフロー分光測定装置を用いた。\n【結果】リン酸緩衝液(0.1 M, pH 7.4)中、DPPHラジカル水溶液にTroloxを加えると、527 nmの吸収が減少し、DPPHラジカルが効率良く消去された。527 nmの吸光度の経時変化を解析することにより、この反応の二次反応速度定数(k)を1.8 × 10(4) M(-1) s(-1)と決定した。この値はエタノール－水(pH 7.4) (1:1 v/v)混合溶媒中における値とよく一致し、β-CDがDPPHラジカルの反応性を阻害しないことが明らかとなった。アスコルビン酸に対しても同様にしてk値を7.2 × 10(3) M(-1) s(-1)と決定した。\n【結論】β-CD包接DPPHラジカルを用いることにより、高濃度(0.1 M)の緩衝液中でも抗酸化物質の活性評価が可能となった。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第68回日本酸化ストレス学会学術集会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中西, 郁夫"}],"nameIdentifiers":[{"nameIdentifier":"646736","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大久保, 敬"}],"nameIdentifiers":[{"nameIdentifier":"646737","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井, 耕平"}],"nameIdentifiers":[{"nameIdentifier":"646738","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"上林, 將人"}],"nameIdentifiers":[{"nameIdentifier":"646739","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉橋, 泰生"}],"nameIdentifiers":[{"nameIdentifier":"646740","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本, 謙一郎"}],"nameIdentifiers":[{"nameIdentifier":"646741","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"福原, 潔"}],"nameIdentifiers":[{"nameIdentifier":"646742","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"寺田, 勝英"}],"nameIdentifiers":[{"nameIdentifier":"646743","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伊東, 忍"}],"nameIdentifiers":[{"nameIdentifier":"646744","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小澤, 俊彦"}],"nameIdentifiers":[{"nameIdentifier":"646745","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"福住, 俊一"}],"nameIdentifiers":[{"nameIdentifier":"646746","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中西 郁夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"646747","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大久保 敬","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"646748","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 耕平","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"646749","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"上林 將人","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"646750","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本 謙一郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"646751","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小澤 俊彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"646752","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"シクロデキストリンで水溶化したDPPHラジカルを用いた水溶性抗酸化物質の速度論的活性評価","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"シクロデキストリンで水溶化したDPPHラジカルを用いた水溶性抗酸化物質の速度論的活性評価"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-05-14"},"publish_date":"2015-05-14","publish_status":"0","recid":"65656","relation_version_is_last":true,"title":["シクロデキストリンで水溶化したDPPHラジカルを用いた水溶性抗酸化物質の速度論的活性評価"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:57:42.845193+00:00"}