{"created":"2023-05-15T14:47:46.682092+00:00","id":65507,"links":{},"metadata":{"_buckets":{"deposit":"bfdbd277-15ec-4f8a-96e4-3e40a715b27e"},"_deposit":{"created_by":1,"id":"65507","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"65507"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00065507","sets":["10:29"]},"author_link":["645212","645216","645217","645213","645215","645214"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2014-09-26","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We previously demonstrated that 2 or 4 Gy of X-ray irradiation induced MIAPaCa-2 invasiveness, whereas 0.5, 1, 2, or 4 Gy of carbon-ion (C-ion) suppressed invasion. ROCK inhibitor in addition to MMP-2 inhibitor was needed to suppress MIAPaCa-2 invasion indicated both mesenchymal and amoeboid modes of motility were functioned. Here we show that 2 Gy of C-ion irradiation reduced GTP-bound Rac1 and RhoA expression, the active form of Rac1 and RhoA, which are the key factors involved in two modes of motility. The reduction of GTP-bound Rac1 or RhoA was recovered by the treatment of proteasome inhibitor, indicated that those proteins were undergo degradation via the ubiquitin-proteasome pathway. So far, IAPs, Inhibitors of Apoptosis Proteins, and HACE1, HECT-domain containing E3 ubiquitin-ligase, were reported as a direct E3 ubiquitin ligase of Rac1. Of those, XIAP was selectively induced after C-ion irradiation. In addition, XIAP was co-precipitated with GTP-bound Rac1 in C-ion irradiated MIAPaCa-2. In conclusion, unlike X-ray irradiation, C-ion irradiation inhibited both mesenchymal and amoeboid motility via GTP-bound Rac1 and RhoA degradation, causing effective reduction of invasion.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第73回日本癌学会学術総会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤田, 真由美"}],"nameIdentifiers":[{"nameIdentifier":"645212","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田, 滋"}],"nameIdentifiers":[{"nameIdentifier":"645213","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井, 高志"}],"nameIdentifiers":[{"nameIdentifier":"645214","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤田 真由美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"645215","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田 滋","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"645216","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 高志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"645217","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"炭素線照射はGTP-bound Rac1 とRhoA のタンパク分解を介し膵癌由来細胞株MIAPaCa-2 の浸潤能を抑制する","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"炭素線照射はGTP-bound Rac1 とRhoA のタンパク分解を介し膵癌由来細胞株MIAPaCa-2 の浸潤能を抑制する"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-09-30"},"publish_date":"2014-09-30","publish_status":"0","recid":"65507","relation_version_is_last":true,"title":["炭素線照射はGTP-bound Rac1 とRhoA のタンパク分解を介し膵癌由来細胞株MIAPaCa-2 の浸潤能を抑制する"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:59:16.728255+00:00"}