{"created":"2023-05-15T14:47:21.904959+00:00","id":64947,"links":{},"metadata":{"_buckets":{"deposit":"045d2ab4-60b5-4022-8abc-d3dbff5835dc"},"_deposit":{"created_by":1,"id":"64947","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"64947"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00064947","sets":["10:29"]},"author_link":["639938","639945","639941","639961","639946","639951","639940","639953","639963","639954","639957","639947","639937","639949","639964","639950","639959","639942","639939","639943","639948","639944","639960","639956","639952","639958","639955","639962"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-03-23","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"移植細胞の動態を経時的に体外から評価することは、移植効果の検討に有用である。良好なMRI造影剤であり細胞内に取り込まれて一定期間保持されるというMn2+の性質に注目し、単核球をマンガン造影剤で標識し、MRIを用い体外評価した。Wistarラット3匹より血液10 mlを採血し、遠心により末梢血単核球を分離した。末梢血単核球は、塩化マンガンを添加した培養液にてincubationにより標識した。中大脳動脈を塞栓子で虚血・再還流した脳虚血モデルラットに、定位脳手術装置を用い穿頭孔を作製し26G穿刺針から標識単核球2μLを線条体部に注射した。小動物用1.5-T MRIとsolenoid MRIコイルを用い、持続吸入麻酔下に撮像を行った。2D Spin echo（SE）法によるT1強調画像（T1W; TR/TE=500/9 ms, FA 90°, FOV＝60×30 mm2, matrix=128×256, voxel size=0.246×0.246×0.246mm3, NEX=8, スライス圧1.0mm）にて、移植直後、12時間後、22時間後に撮像した。移植直後は投与部に集簇している標識細胞を経時的に観察すると、対照群では同心円状に広がるが、脳虚血群では脳虚血部方向への広がりが早かった。平均血圧が移植後2週間に比べ3週間で有意に上昇し、対照群レベルとなった。末梢血単核球にマンガン標識し経時的にMRIで撮像することにより、脳病変においても移植細胞の動態評価が可能であった。使用したマンガンの総投与量は微量であり、動物実験のみならず、安全性を確立した後には臨床にも有用と期待される。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第12回日本再生医療学会総会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only 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