{"created":"2023-05-15T14:47:13.128308+00:00","id":64752,"links":{},"metadata":{"_buckets":{"deposit":"b607f391-291d-4194-9c3e-facc05c342eb"},"_deposit":{"created_by":1,"id":"64752","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"64752"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00064752","sets":["10:29"]},"author_link":["638008","638007","638010","638009","638005","638006"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-09-21","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Tumor cells invade by two modes of motility-mesenchymal and amoeboid. Irradiation alters the invasiveness of several tumor cell lines, however the effects on the modes of motility remain unknown. Here we report that X-ray irradiation enhanced MIAPaCa-2 and PANC-1 invasion via MMP-2, whereas, Carbon-ion irradiation reduced MIAPaCa-2 invasion but increased PANC-1 invasion via serine proteases (SerP). Treatment of MMP inhibitor or SerP inhibitor failed to decrease the radiation-enhanced MIAPaCa-2 or PANC-1 invasion accompanied by mesenchymal-amoeboid transition. ROCK inhibitor plus those protease inhibitor suppressed the enhanced invasiveness, suggested that both modes of motility were significant in MIAPACa-2 and PANC-1. We further found that irradiation affects the activity of small GTPase, Rac1 and RhoA, the key factors involved in two modes of motility. Rac1 was activated in X-ray-irradiated MIAPaCa-2, whereas, both Rac1 and RhoA activities were diminished in C-ion-irradiated MIAPaCa-2. In contrast, RhoA was activated in C-ion-irradiated PANC-1. These results suggested that irradiation alters the invasive potential through protease activity, and also Rac1 and RhoA activities.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"The 71st Annual Meeting of the Japanese Cancer Association (JCA)","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fujita, Mayumi"}],"nameIdentifiers":[{"nameIdentifier":"638005","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, Shigeru"}],"nameIdentifiers":[{"nameIdentifier":"638006","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"638007","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤田 真由美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"638008","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田 滋","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"638009","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 高志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"638010","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Irradiation alters the invasive potential of human pancreatic cancer cell lines thorough Rac1 and RhoA activities","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Irradiation alters the invasive potential of human pancreatic cancer cell lines thorough Rac1 and RhoA activities"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-09-24"},"publish_date":"2012-09-24","publish_status":"0","recid":"64752","relation_version_is_last":true,"title":["Irradiation alters the invasive potential of human pancreatic cancer cell lines thorough Rac1 and RhoA activities"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:08:02.227334+00:00"}