@misc{oai:repo.qst.go.jp:00064752,
 author = {Fujita, Mayumi and Yamada, Shigeru and Imai, Takashi and 藤田 真由美 and 山田 滋 and 今井 高志},
 month = {Sep},
 note = {Tumor cells invade by two modes of motility-mesenchymal and amoeboid. Irradiation alters the invasiveness of several tumor cell lines, however the effects on the modes of motility remain unknown. Here we report that X-ray irradiation enhanced MIAPaCa-2 and PANC-1 invasion via MMP-2, whereas, Carbon-ion irradiation reduced MIAPaCa-2 invasion but increased PANC-1 invasion via serine proteases (SerP). Treatment of MMP inhibitor or SerP inhibitor failed to decrease the radiation-enhanced MIAPaCa-2 or PANC-1 invasion accompanied by mesenchymal-amoeboid transition. ROCK inhibitor plus those protease inhibitor suppressed the enhanced invasiveness, suggested that both modes of motility were significant in MIAPACa-2 and PANC-1. We further found that irradiation affects the activity of small GTPase, Rac1 and RhoA, the key factors involved in two modes of motility. Rac1 was activated in X-ray-irradiated MIAPaCa-2, whereas, both Rac1 and RhoA activities were diminished in C-ion-irradiated MIAPaCa-2. In contrast, RhoA was activated in C-ion-irradiated PANC-1. These results suggested that irradiation alters the invasive potential through protease activity, and also Rac1 and RhoA activities., The 71st Annual Meeting of the Japanese Cancer Association (JCA)},
 title = {Irradiation alters the invasive potential of human pancreatic cancer cell lines thorough Rac1 and RhoA activities},
 year = {2012}
}