{"created":"2023-05-15T14:47:09.290224+00:00","id":64665,"links":{},"metadata":{"_buckets":{"deposit":"165cefdc-8c2c-4180-ba6e-8b19ec6d0fd3"},"_deposit":{"created_by":1,"id":"64665","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"64665"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00064665","sets":["10:29"]},"author_link":["637286","637283","637285","637279","637277","637280","637278","637282","637281","637284"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-06-29","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"膵臓癌は悪性度が高く化学療法や一般放射線治療に抵抗性を示し、依然代表的な難治がんである。本研究は、膵臓癌細胞株PANC1、PK45を用い、放射線抵抗性や薬剤耐性と強く関与するとされる癌幹細胞を分離・同定し、これら癌幹細胞に対して、炭素線或いはX線照射前後のコロニー形成能、spheroid形成能、DNA損傷の違いを調べ、またSCIDマウスに移植し、腫瘍形成能、移植腫瘍に対する増殖抑制や治癒率の違いについて比較検討した。10％生存線量（D10）におけるX線に対する炭素線の生物学的効果比（RBE）は1.71-2.18であった。PANC1、PK45細胞においてCD44+/CD24+、CD44+/ESA+細胞はCD44-/CD24-、CD44-/ESA-細胞に比べ有意にコロニー形成数が多く、spheroid形成や腫瘍形成はCD44+/CD24+、CD44+/ESA+細胞のみに認められた。また、癌幹細胞におけるDNA損傷マーカーH2AX foci数の経時的変化では、X線に比べ炭素線照射後より多くの残存が認められた。CD44+/CD24+、CD44+/ESA+細胞は、X線、炭素線照射に対しともに抵抗性を示すが、炭素線はより強い細胞殺傷能力が認められた。炭素線はX線照射に比べより強い腫瘍増殖抑制や高い治癒率が認められた。以上より、膵臓癌細胞において、CD24+/CD44+、Cd44+/ESA+細胞は明らかに自己複製や放射線抵抗性を示しており、炭素線はX線照射に比べより強く膵臓癌幹細胞を殺傷することが示唆された。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第42回日本膵臓学会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"崔, 星"}],"nameIdentifiers":[{"nameIdentifier":"637277","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大西, 和彦"}],"nameIdentifiers":[{"nameIdentifier":"637278","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"上條, 岳彦"}],"nameIdentifiers":[{"nameIdentifier":"637279","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田, 滋"}],"nameIdentifiers":[{"nameIdentifier":"637280","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鎌田, 正"}],"nameIdentifiers":[{"nameIdentifier":"637281","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"637282","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大西 和彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"637283","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"上條 岳彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"637284","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田 滋","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"637285","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鎌田 正","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"637286","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"重粒子線照射によるヒト膵癌幹細胞生存率、DNA修復及び移植腫瘍への影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"重粒子線照射によるヒト膵癌幹細胞生存率、DNA修復及び移植腫瘍への影響"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-07-11"},"publish_date":"2012-07-11","publish_status":"0","recid":"64665","relation_version_is_last":true,"title":["重粒子線照射によるヒト膵癌幹細胞生存率、DNA修復及び移植腫瘍への影響"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:09:01.956261+00:00"}