{"created":"2023-05-15T14:47:08.333169+00:00","id":64644,"links":{},"metadata":{"_buckets":{"deposit":"ab6009c5-42f5-4a18-871d-163ab0f5cbf3"},"_deposit":{"created_by":1,"id":"64644","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"64644"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00064644","sets":["10:29"]},"author_link":["637055","637069","637065","637058","637067","637054","637068","637060","637061","637057","637056","637053","637063","637059","637062","637066","637064"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-06-14","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"移植細胞の動態を体外から評価することは、移植効果の検討にも有用\nである。良好なMRI 造影剤であり細胞内に取り込まれて一定期間保持\nされるというMn2+ の性質に注目し、単核球をマンガン造影剤で標識し、\nMRI を用い体外評価した。Wistar ラットを対象とし、採血用Wistar ラッ\nト3 匹より血液10 ml を採血し、遠心により末梢血単核球を分離した。\n単核球は、塩化マンガンを添加した培養液にてincubation により標識し\nた。2% イソフルラン持続吸入麻酔下にラットに定位脳手術装置を用い、\n穿頭孔を作製し26G 穿刺針から標識単核球2 μ L を線条体部に注射し\nた。小動物用1.5-T MRI とsolenoid MRI コイルを用い、持続吸入麻酔\n下に撮像を行った。2D Spin echo（SE）法によるT1 強調画像（T1W;\nTR/TE = 500/9 ms, FA 90°, FOV ＝ 60 × 30 mm2）にて冠状断面を\n得た。直後、12 時間後、22 時間後、36 時間後に撮像した。移植直後に\nは投与部に集簇していた標識細胞が、経時的に投与部から正円状に拡\n散していく様子が観察された。移植細胞が少ないと、投与直後には観察\nされた標識細胞が12 時間後からは観察できなくなった。マンガン造影\n剤の移植部への沈着の影響は少ないと考えられた。マンガン標識とMRI\nにより、脳での移植細胞の新しい動態評価法が示された。使用したマン\nガンの総投与量は微量であり、動物実験のみならず、安全性を確立した\n後には臨床にも有用と期待される。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第11回日本再生医療学会総会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小高, 謙一"}],"nameIdentifiers":[{"nameIdentifier":"637053","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伊藤, 康一"}],"nameIdentifiers":[{"nameIdentifier":"637054","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"青木, 伊知男"}],"nameIdentifiers":[{"nameIdentifier":"637055","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"島田, 拓也"}],"nameIdentifiers":[{"nameIdentifier":"637056","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森谷, 純治"}],"nameIdentifiers":[{"nameIdentifier":"637057","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"舘野, 馨"}],"nameIdentifiers":[{"nameIdentifier":"637058","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"南野, 徹"}],"nameIdentifiers":[{"nameIdentifier":"637059","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小室, 一成"}],"nameIdentifiers":[{"nameIdentifier":"637060","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"下山, 一郎"}],"nameIdentifiers":[{"nameIdentifier":"637061","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀, 恒夫"}],"nameIdentifiers":[{"nameIdentifier":"637062","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"福村, 利光"}],"nameIdentifiers":[{"nameIdentifier":"637063","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小高 謙一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"637064","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"青木 伊知男","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"637065","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森谷 純治","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"637066","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"舘野 馨","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"637067","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀 恒夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"637068","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"福村 利光","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"637069","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"ラット脳への末梢血単核球移植後の動態評価：マンガン標識とＭＲＩ（磁気共鳴画像）","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ラット脳への末梢血単核球移植後の動態評価：マンガン標識とＭＲＩ（磁気共鳴画像）"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-06-29"},"publish_date":"2012-06-29","publish_status":"0","recid":"64644","relation_version_is_last":true,"title":["ラット脳への末梢血単核球移植後の動態評価：マンガン標識とＭＲＩ（磁気共鳴画像）"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:09:16.243932+00:00"}