{"created":"2023-05-15T14:46:58.380306+00:00","id":64441,"links":{},"metadata":{"_buckets":{"deposit":"4aa93af3-874a-48e9-a232-3634c88b29be"},"_deposit":{"created_by":1,"id":"64441","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"64441"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00064441","sets":["10:29"]},"author_link":["635343","635345","635342","635344","635346","635348","635341","635347"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2011-11-19","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"肝細胞癌は世界で最も罹患数が多い悪性腫瘍の一つで、日本では増加傾向であり、年間死亡数は3.4万人とがん死亡では３位である。放医研では今まで300例近い重粒子線による肝癌治療を行っており、良好な治療成績が得られている。本研究は、肝癌細胞株Huh7、HepG2を用い、放射線抵抗性や薬剤耐性と強く関与するとされる癌幹細胞を分離・同定し、これら癌幹細胞に対して、炭素線或いはX線照射前後のコロニー形成能、spheroid形成能、DNA損傷の違いを調べ、またSCIDマウスに移植し、腫瘍形成能の違いについて比較検討した。Huh7、HepG2細胞においてCD133+/CD90+はそれぞれ6.4％と0.6％、CD44+/ESA+細胞は1.5％と0.2％であった。CD133+/CD90+、CD44+/ESA+細胞はCD133-/CD90-、CD44-/ESA-細胞に比べ有意にコロニー形成数が多く、spheroid形成や腫瘍形成はCD133+/CD90+、CD44+/ESA+細胞のみに認められた。CD133+/CD90+、CD44+/ESA+細胞は、X線、炭素線照射に対しともに抵抗性を示すが、炭素線はより強い細胞殺傷能力が認められた。炭素線はX線照射に比べより強い腫瘍増殖抑制や高い治癒率が認められた。以上より、肝癌細胞において、CD133+/CD90+、CD44+/ESA+細胞は明らかに自己複製や放射線抵抗性を示しており、炭素線はX線照射に比べより強く肝癌幹細胞を殺傷することが示唆された。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会第54回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"崔, 星"}],"nameIdentifiers":[{"nameIdentifier":"635341","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大西, 和彦"}],"nameIdentifiers":[{"nameIdentifier":"635342","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田, 滋"}],"nameIdentifiers":[{"nameIdentifier":"635343","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鎌田, 正"}],"nameIdentifiers":[{"nameIdentifier":"635344","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"635345","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大西 和彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"635346","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田 滋","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"635347","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鎌田 正","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"635348","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"ヒト肝細胞癌における癌幹細胞の同定及びX線や重粒子線照射による影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒト肝細胞癌における癌幹細胞の同定及びX線や重粒子線照射による影響"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-12-01"},"publish_date":"2011-12-01","publish_status":"0","recid":"64441","relation_version_is_last":true,"title":["ヒト肝細胞癌における癌幹細胞の同定及びX線や重粒子線照射による影響"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:11:35.176256+00:00"}