@misc{oai:repo.qst.go.jp:00064039,
 author = {Fujita, Hidetoshi and Ohkubo, Yu and Iwakawa, Mayumi and Seino, Ken-ichiro and Nakano, Takashi and Imai, Takashi and 藤田 英俊 and 大久保 悠 and 岩川 眞由美 and 清野 研一郎 and 中野 隆史 and 今井 高志},
 month = {Sep},
 note = {Purpose:Our previous research suggested carbon-ion (c-ion) irradiation upregulated immunogenicity in irradiated tumor, underlining the potential clinical application of radioimmunotherapy. The anti-metastatic mechanism of this therapy was examined using in vivo murine model.
Methods:Local leg tumors were inoculated with squamous cell carcinoma (NR-S1) in C3H mice, or Lewis lung carcinoma (LLC) in C57BL and NKT knockout mice. Local tumors were irradiated with a single dose of 6 Gy of c-ion. Thirty-six hours after irradiation, alpha-GalCer-pulsed dendritic cells (alpha-GalCer-DCs) were injected into the leg tumor. The effects on lung metastasis were investigated by analysis of S100A8, a marker of lung pre-metastatic phase.
Results:Local tumors inoculated with NR-S1 or LLC in the leg were spontaneously spread to lung, and formed several metastatic nodules. Metastatic nodules of NR-S1 were decreased in the mice treated with combination therapy. The expression of S100A8 was reduced only in the combination of c-ion and alpha-GalCer-DCs.
Conclusion:Our research suggested a possible mechanism that niche status of distant organ might be regulated by local c-ion irradiated tumor with enhanced immunity., 69th Annual Meeting of Japanese Cancer Association},
 title = {Anti-metastatic efficacy in carbon-ion radiotherapy combined with alpha--GalCer-pulsed dendritic cells},
 year = {2010}
}