@misc{oai:repo.qst.go.jp:00064027,
 author = {長谷川, 純崇 and 長谷川 純崇},
 month = {Sep},
 note = {Modeling cancer in laboratory animal is important for elucidating molecular mechanism of tumor formation and is critical for the development of effective therapy against cancer. The recent success with small fish as an animal model of cancer has attracted cancer researchers attention because of the capability of directly visualizing tumor cells in vivo. We generated a unique medaka model, in which we are able to observe various cell behaviors of in vivo transplanted tumor cells such as cell proliferation and metastasis. First, we established medaka melanoma cells stably expressing green fluorescence protein (GFP) and transplanted them into non-irradiated and irradiated medaka. The tumor cells were grown at the injection sites in medaka, and the spatiotemporal changes were visualized under fluorescence stereoscopic microscope with a cellular level-resolution, even at a single cell level. Interestingly, in irradiated medaka, accelerated tumor growth and metastasis of the transplanted tumor cells were directly visualized. Our medaka model would provide a powerful tool for in vivo tumor cell biology, allowing direct observation of in vivo tumor cells as seen in a culture dish., 第69回日本癌学会学術総会},
 title = {メダカを用いたがん動物モデルの作製とそのがん生物学への展開},
 year = {2010}
}