@misc{oai:repo.qst.go.jp:00063774,
 author = {江口−笠井, 清美 and 辻田, 瑛那 and 森, 雅彦 and 笠井 清美 and 辻田 瑛那 and 森 雅彦},
 month = {Apr},
 note = {We studied the localization of phosphorylated H2AX (gamma-H2AX) in cultured human fibroblasts (NB1RGB) and YGFP-tagged rad51 in Chinese hamster CHO cells after irradiation with heavy ion beams. Asynchronous cells were irradiated with X-rays, carbon ion beam (LET is about 30 keV/um and 85-90 keV/um), Si ion beam (220 keV/um) and Fe ion beam (440 keV/um) at room temperature. The amount of gamma-H2AX monitored by flow cytometry increased just after irradiation of each radiation and reached maximum around 30 min. Amount of gamma-H2AX per cells at 30 min after irradiation increased with dose for all radiations tested and the radiation with higher LET induced gamma-H2AX more effectively than those with low LET. Under the confocal microscopy, foci of gamma-H2AX on cell nucleus was not visible just after X-irradiation, but obvious after Fe ion irradiation.
Number of foci per nucleus was increased with increasing dose at 30 min after irradiation. However, low LET carbon is more effective than Fe for foci induction. On the other hand, rad51 foci were not visible just after irradiation and observed about 40 min after irradiation and were visible up to 10 h after irradiation. The cells in which there was no focus at early incubation did not have the focus after longer incubation., 平成20年度放射線医学総合研究所重粒子線がん治療装置等共同利用研究成果発表会},
 title = {重粒子線誘発DNA 障害の特徴},
 year = {2009}
}