@misc{oai:repo.qst.go.jp:00063623,
 author = {長谷川, 正俊 and 浅川, 勇雄 and 玉本, 哲郎 and 石内, 勝吾 and 新, 雅子 and 大野, 達也 and 櫻井, 英幸 and 田巻, 倫明 and 加藤, 真吾 and 中野, 隆史 and 村上, 健 and 浅川 勇雄 and 大野 達也 and 田巻 倫明 and 加藤 眞吾 and 中野 隆史 and 村上 健},
 month = {Apr},
 note = {The aim of this study is to investigate the radiobiological effect of heavy ion beames on intratumoral radioresistant and radiosensitive human tumor cells. Tumors of human origin, an ependymoblastoma with wild-type (wt) p53, a primitive neuroectodermal tumor with wt p53, and a glioblastoma with mutant-type (mt) p53, were transplanted to nude mice, and the mice were irradiated with carbon ion beams (290MeV/u, 6 cm spread-out Bragg peak) or 200kV X-rays. Tumors were excised 4, 6, or 24 hours after 2Gy irradiation. A part of each tumor was fixed in formalin and embedded in paraffin. Thin sections were stained with H.E., p53, Ki-67, GFAP or TUNEL for microscopic study. The other part of each tumor was stored in RNA stabilization solution, and total RMA was extracted for cDNA microarray analysis. In p53 wt tumors, apoptosis increased 4 and 6 hours after irradiation, and then GFAP-positive cells increased. Less histological changes were observed in a p53 mt tumor. The tumors with wt p53 showed significant changes in gene expression following irradiation. Pathway analysis of up- and down-regulated genes demonstrated that apoptosis, p53 signaling pathway, and cell cycle are involved significantly. There was little difference between the gene expression profiles induced by carbon ion beams and those by X-rays, but the extent of up- or down-regulation was more evident after carbon ion irradiation than after X-rays. In contrast, the tumor with mt p53 showed much less change in gene expression after irradiation., H20年度HIMAC共同利用研究 成果発表会},
 title = {腫瘍内の放射線抵抗性細胞に対する重粒子線の効果と検討},
 year = {2009}
}