{"created":"2023-05-15T14:46:20.074577+00:00","id":63605,"links":{},"metadata":{"_buckets":{"deposit":"00b7cba8-db93-44a5-84e2-4092b487c531"},"_deposit":{"created_by":1,"id":"63605","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"63605"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00063605","sets":["10:29"]},"author_link":["627715","627718","627717","627720","627719","627723","627724","627716","627721","627725","627722"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2009-10-03","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"【目的】hNISレポーター遺伝子を安定発現する癌細胞株を作製し、ヌードマウスに移植し形成した腫瘍をSPECT/CTで撮像した。【方法】pcDNA3-hNISをヒト大腸癌細胞株HCT116に導入して安定発現株を作製し、うち３系統（A、B、E）と陰性対照（N）を、16匹のヌードマウスの四肢へ皮下移植した。8mm以上の腫瘍の形成率と形成日数を調べ、小動物用SPECT/CTシステムFX（Gamma Medica-Ideas）を用いて99mTcO4- (Tc) SPECTを行った。【結果】腫瘍形成率は、系統Aが69％、Bが63％、Eが94％、対照Nが88％（p＝NS）、形成日数は、Aが36±9日、Bが24±8日、Eが26±12日、Nが18±12日（A対Nがp＜0.05、他はp＝NS）、腫瘍Tc集積（％ID/g）は、Aが25.7±8.3、Bが35.9±12.3、Eが11.6±6.4、Nが2.4±0.9だった（A対NとB対Nがp＜0.001、B対Eがp＜0.01、他はp＝NS）。【結論】腫瘍形成能の低下がなくTc集積が最も高い系統Bが最適な安定発現株と考えられた。この細胞株を用いれば、将来マウス生体内で癌転移の過程を追跡できる可能性があり、癌転移の分子機構解明に役立つと期待される。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第49回日本核医学会学術総会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"村井, 知佳"}],"nameIdentifiers":[{"nameIdentifier":"627715","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"犬伏, 正幸"}],"nameIdentifiers":[{"nameIdentifier":"627716","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"志田原, 美保"}],"nameIdentifiers":[{"nameIdentifier":"627717","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻, 厚至"}],"nameIdentifiers":[{"nameIdentifier":"627718","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"北川, 善政"}],"nameIdentifiers":[{"nameIdentifier":"627719","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀, 恒夫"}],"nameIdentifiers":[{"nameIdentifier":"627720","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"村井 知佳","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"627721","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"犬伏 正幸","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"627722","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"志田原 美保","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"627723","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 厚至","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"627724","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀 恒夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"627725","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"ヒトNa＋/I−共輸送蛋白（hNIS）遺伝子安定発現大腸癌細胞株マウス担癌モデルのレポーター遺伝子イメージング","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒトNa＋/I−共輸送蛋白（hNIS）遺伝子安定発現大腸癌細胞株マウス担癌モデルのレポーター遺伝子イメージング"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-10-06"},"publish_date":"2009-10-06","publish_status":"0","recid":"63605","relation_version_is_last":true,"title":["ヒトNa＋/I−共輸送蛋白（hNIS）遺伝子安定発現大腸癌細胞株マウス担癌モデルのレポーター遺伝子イメージング"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:21:04.463096+00:00"}