{"created":"2023-05-15T14:46:19.005928+00:00","id":63581,"links":{},"metadata":{"_buckets":{"deposit":"3c508dfa-3309-4ae6-b891-b91177c1935a"},"_deposit":{"created_by":1,"id":"63581","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"63581"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00063581","sets":["10:29"]},"author_link":["627522","627526","627532","627517","627527","627521","627533","627536","627518","627534","627523","627524","627520","627525","627529","627535","627531","627528","627530","627519"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-11-21","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"低線量放射線の生物学的影響を探索する目的で、低線量率ガンマ線長期照射によるC57BL/6雄マウス肝臓での遺伝子発現レベルの変化をAffymetrix社Mouse Genome 430 2.0 アレイにより解析した。ほぼ等しい線量率で照射実験を二回行った。一回目は照射線量率を32 nGy/min（低線量率）、650 nGy/min（中線量率）、12500 nGy/min（高線量率）として485日間照射（総照射線量はそれぞれ20.6 mGy, 414 mGy, 8000 mGy）、二回目は線量率を38 nGy/min（低）、767 nGy/min（中）、15300 nGy/min（高）として410日間照射（総線量は20 mGy, 401 mGy, 8015 mGy）した。照射後すぐにマウスを屠殺し、各照射線量率一群３匹（二回の照射のため各群計６匹）の個体より肝臓を摘出し、Total RNAを抽出した。対照群は３匹（一回目）、６匹（二回目）のRNAを等量ずつプールしプローブとして使用した。解析の結果、個体ごとに発現レベルが大きく異なる遺伝子はかなり多く、放射線照射に応答して変動する遺伝子の数は多くはなかった。Wilcoxon’s Signed Rank testに基づいた発現比較アルゴリズムを利用し、６個体全てで対照群に対して１.５倍以上発現が変動していると判定された遺伝子数は低線量率群、中線量率群、高線量率群でそれぞれ２、１５、２５個であり、そのほとんどの遺伝子の発現変動はRT-PCRにより確認できた。今回、最も低い線量率でも２個の遺伝子で２倍以上の発現変化が見られたことで、非常に低線量率の放射線に対しても応答する遺伝子が存在することがわかった。さらに、Ingenuity Pathways Analysisを用いた解析からは、高線量率で発現変動が見られた遺伝子群で脂質代謝、肥満などの機能に影響を与える可能性が示唆された。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会　第５１回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only 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