@misc{oai:repo.qst.go.jp:00063577,
 author = {柏倉, 幾郎 and 高橋, 賢次 and 門前, 暁 and 吉野, 浩教 and 阿部, 由直 and 江口−笠井, 清美 and 柏倉 幾郎 and 高橋 賢次 and 門前 暁 and 吉野 浩教 and 阿部 由直 and 笠井 清美},
 month = {Apr},
 note = {To demonstrate the effects of high and low
LET-radiation on human megakaryocyte
development and platelet production, the gene
expressions of hematopoietic stem/progenitor cells
exposed to heavy ion beams and X-rays were
analyzed.
CD34+ cells derived from placental and umbilical
cord blood were induced by carbon-ion beams
(LET = 50 keV) or X-rays at 2 Gy, differentiated
into megakaryocytes and platelets with
thrombopoietin (TPO) and interleukin-3 (IL-3) for
7 days and then assayed using real time RT-PCR.
FLI1 and RUNX1, positive regulators of
megakaryocytopoiesis, were up-regulated by the
exposure to carbon-ion beams. The expressions of
MPL, ITGA2B and GPIBA, the
megakaryocyte-specific genes, were reduced by
X-irradiation. In other factors, X-rays increased the
expression of KIT, the receptor for SCF while
carbon-ion beams enhanced the expression of
C/EBPA, a myeloid transcription factor.
These results showed that the regulation of several
gene expressions were dependent on each radiation,
respectively. The relations of these molecules on
human megakaryocytopoiesis and thrombopoiesis
remain to be examined., 平成19年度放射線医学総合研究所重粒子線がん治療装置等共同利用研究成果発表会},
 title = {ヒト巨核球・血小板造血過程における遺伝子発現の変化と放射線の影響},
 year = {2008}
}