{"created":"2023-05-15T14:46:06.966496+00:00","id":62994,"links":{},"metadata":{"_buckets":{"deposit":"d17dfe23-dcff-4119-aacf-ccacea23335a"},"_deposit":{"created_by":1,"id":"62994","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"62994"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00062994","sets":["10:29"]},"author_link":["622184","622178","622192","622183","622190","622176","622177","622198","622196","622185","622199","622186","622195","622193","622179","622181","622188","622197","622189","622182","622187","622175","622194","622180","622191"],"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"遺伝子組換えによって緑色蛍光タンパク質EGFPをほとんどの体細胞において発現するGFP-マウスの利用は生物研究に大きな貢献を果たしている。しかし、２種類あるいは３種類の細胞間の相互作用を調べる為にはGFP-マウスに加え、第二の蛍光マウスが必要になる。青色蛍光タンパク質ECFP（励起433nm/ 蛍光475nm）はEGFP（488/509）と比べ蛍光が微弱、かつ、一般的な細胞解析装置での測定が出来ないため、利用しにくい欠点があったが、蛍光顕微鏡の画像解析システムにCFP用フィルターを加えて２つの蛍光を区別して測定することが可能になった。今回、ジャクソンよりB6.129(ICR)-Tg(ACTB-ECFP)CK6Nagy/J（CFP-マウスと略す）を輸入し、クレアのB６マウスに３代バッククロスののち帝王切開法で清浄化してSPF動物生産実験棟２階に導入した。動物導入の経緯とこれらマウスを用いた研究例を報告する。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第4回技術と安全の報告会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石井, 洋子"}],"nameIdentifiers":[{"nameIdentifier":"622175","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻, 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大介","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"622197","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伊藤 正人","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"622198","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石原 直樹","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"622199","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference 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