{"created":"2023-05-15T14:46:03.947126+00:00","id":62927,"links":{},"metadata":{"_buckets":{"deposit":"b4bcd2d5-80f6-4c75-8560-35c29fdb88d8"},"_deposit":{"created_by":1,"id":"62927","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"62927"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00062927","sets":["10:29"]},"author_link":["621625","621620","621626","621615","621622","621617","621618","621619","621624","621616","621623","621621"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-12-17","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"DNA二本鎖切断（DSB）は放射線の最も重大な生物影響のひとつである。高等真核生物では，相同組換（HR）と非相同末端結合（NHEJ）の２つのDSB修復機構が知られている。細胞周期S期後期からG2期に特異的なHRに対して，細胞周期に依存しないNHEJは，高等真核生物の主要なDSB修復経路と考えられている。ニワトリやマウスの遺伝子改変細胞，遺伝子改変動物を含む多様な実験系での解析から，今までに，NHEJに関わる７つのタンパク質（Ku70，Ku80，DNA-PKcs，Artemis，DNA ligase IV，XRCC4，XLF/Cernunnos）が同定され，その機能の概要が明らかになってきている。しかしながら，ヒトのNHEJにおけるこれらのタンパク質の機能についての情報は，未だ不足している。我々はヒトの放射線リスクにおけるNHEJの関与を明らかにすることを目的に研究を行っている。先頃，ヒト大腸癌由来で，正常に近い核型を保持しているHCT116培養細胞株を用いて，特定の遺伝子を高効率に破壊する方法を確立した。これにより，遺伝的背景が同じヒトの細胞で，特定の遺伝子機能の解析が可能になった。今回のシンポジウムでは，HCT116から樹立したArtemis-/-細胞株，XRCC4-/-細胞株について，種々のDNA損傷誘導要因（放射線，DNA損傷誘導薬剤，活性酸素など）に対する感受性を，生存率，染色体異常，ヒストンH2AXのリン酸化等を指標に検討した結果を報告する。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第3回放射線防護研究センターシンポジウム","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"勝部, 孝則"}],"nameIdentifiers":[{"nameIdentifier":"621615","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森, 雅彦"}],"nameIdentifiers":[{"nameIdentifier":"621616","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻, 秀雄"}],"nameIdentifiers":[{"nameIdentifier":"621617","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見, 尚子"}],"nameIdentifiers":[{"nameIdentifier":"621618","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見, 忠博"}],"nameIdentifiers":[{"nameIdentifier":"621619","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小野田, 眞"}],"nameIdentifiers":[{"nameIdentifier":"621620","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"勝部 孝則","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"621621","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森 雅彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"621622","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 秀雄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"621623","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見 尚子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"621624","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見 忠博","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"621625","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小野田 眞","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"621626","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"DNA修復遺伝子欠損ヒト細胞の放射線および薬剤に対する感受性","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"DNA修復遺伝子欠損ヒト細胞の放射線および薬剤に対する感受性"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-12-18"},"publish_date":"2008-12-18","publish_status":"0","recid":"62927","relation_version_is_last":true,"title":["DNA修復遺伝子欠損ヒト細胞の放射線および薬剤に対する感受性"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:25:09.198575+00:00"}