@misc{oai:repo.qst.go.jp:00062679,
 author = {柳澤, 充 and 盛武, 敬 and 中渡, 美也子 and 今留, 香織 and 酒井, 美奈子 and 中村, 悦子 and 岩川, 眞由美 and 今井, 高志 and 柳澤 充 and 盛武 敬 and 中渡 美也子 and 今留 香織 and 酒井 美奈子 and 中村 悦子 and 岩川 眞由美 and 今井 高志},
 month = {Apr},
 note = {Purpose---To clarify the molecular mechanism of radiation induced late
injury in normal tissue, using murine model. In the first year of this
study, aiming to irradiate the lung or the urinary bladder of mice with
carbon-ion, new type of collimators was constructed.
Materials and methods --- C3H/HeNrs and C57BL/6J, 8-week-old female mice
were used through experiments. The position of the target, either lung or
bladder, was confirmed by x-ray photographs. Also, the absorbed dose at the
target was confirmed using photoluminescence dosimeter (PLD) and ionization
chamber. Exposures were conducted using horizontal carbon-12 beams with 290
MeV/u at a dose rate of approximately 3 Gy/min within the 6-cm spread-out
bragg peak (SOBP). Gamma-rays were used as reference beam with new
collimators. To evaluate the effects of C-ion irradiation on lungs,
pathological changes and immunohistochemical stainings for CD44 and Bak at 6
hr, day16, 28 and 56 after thoracic irradiation were analyzed. To evaluate
the adverse effects on the bladder,cystometry and pathological evaluation
were performed.The total RNA was extracted from irradiated tissues, which
were removed on day1,4,8,15,29,60,90,150,240 and 360 after irradiation for
microarray analysis to elucidate the molecular response to irradiation.
Results --- The number of CD44 and Bak positive cells in the irradiated lungs
were changed after irradiation. The capacity of the urinary bladder and the
compliance were decreased significantly at day1,4,8 and 29 after
irradiation., H19年度HIMAC共同利用研究成果発表会},
 title = {晩期障害マウスモデルを用いた重粒子線治療有害事象の検討},
 year = {2008}
}