{"created":"2023-05-15T14:45:43.798686+00:00","id":62480,"links":{},"metadata":{"_buckets":{"deposit":"2d731a93-6391-4421-8956-d5d13bea6dc4"},"_deposit":{"created_by":1,"id":"62480","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"62480"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00062480","sets":["10:29"]},"author_link":["617322","617321","617317","617319","617316","617315","617323","617324","617320","617318"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-03-22","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A central paradigm in radiation biology has been that only a cell hit by a track of radiation would be affected to induce radiobiological effects, and a cell not hit should not be. This paradigm is the basis for the current system for risk estimation from radiation. However, it recently has been challenged by so called non-targeted effects, including bystander effect and genomic instability, and such radiation-induced non-targeted effects may have important implications for risk evaluation of low dose / low dose rate radiations. In this study we have investigated cellular responses in normal human fibroblasts induced with low dose / low dose rate irradiations of qualitatively different radiation types, such as gamma rays, neutrons and high-LET heavy ions. Cells were pre-treated with low-fluence irradiation (~1mGy/7-8h) of 137Cs gamma rays, 241Am-Be neutrons, helium ions (LET=2.3keV/µm) and carbon ions (LET=13.3keV/µm) before following irradiation with a 200kV X-ray challenge dose. The helium- and carbon-ion beams were produced by the Heavy Ion Medical Accelerator in Chiba (HIMAC) at National Institute of Radiological Sciences in Japan. There was observed no difference in X-ray induced cell-killing effects, which was detected with the colony-forming assay, when using pre-treatment with low-dose irradiations of gamma rays, neutrons, helium ions and carbon ions. For mutation induction at hprt locus detected as 6-thioguanine resistant clones, there was no difference in X-ray-induced mutation frequency at 1.5Gy of X-ray challenge dose between un-pretreated and gamma-ray pre-treated cells. In the case of the pre-treatment with high-LET ions, mutation frequency was around 4.0 times higher in carbon-ion pre-treated cells and 1.9 times higher in helium-ion pre-treated cells than in un-pretreated cells. On the contrary, it was reduced in neutron pre-treated cells, when comparing to un-pretreated cells. Furthermore, the enhancement of X-ray-induced mutation frequency in cells pre-treated with helium and carbon ions was reduced at the control level, when using a specific inhibitor of gap-junction mediated cell-cell communication (40µM lindane). There is evidence that gap-junction mediated cell-cell communication play an important role of inducing genomic instability by pre-treatment with heavy ions.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"Heavy Ion Research Joint Meeting","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Suzuki, Masao"}],"nameIdentifiers":[{"nameIdentifier":"617315","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Uchihori, Yukio"}],"nameIdentifiers":[{"nameIdentifier":"617316","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kitamura, Hisashi"}],"nameIdentifiers":[{"nameIdentifier":"617317","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuruoka, Chizuru"}],"nameIdentifiers":[{"nameIdentifier":"617318","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"617319","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木 雅雄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"617320","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"内堀 幸夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"617321","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"北村 尚","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"617322","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鶴岡 千鶴","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"617323","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"617324","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Radiation-quality dependence of genomic instability in mutation induced by the pre-treatment with low-fluence heavy ions.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Radiation-quality dependence of genomic instability in mutation induced by the pre-treatment with low-fluence heavy ions."}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-03-25"},"publish_date":"2008-03-25","publish_status":"0","recid":"62480","relation_version_is_last":true,"title":["Radiation-quality dependence of genomic instability in mutation induced by the pre-treatment with low-fluence heavy ions."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:30:18.392796+00:00"}