@misc{oai:repo.qst.go.jp:00062365,
 author = {Akino, Yuichi and Inaoka, Miho and Furusawa, Yoshiya and et.al and 秋野 祐一 and 稲岡 美穂 and 古澤 佳也},
 month = {Sep},
 note = {Purpose:  Both surgery and radiotherapy have limitation to treat metastasized malignant tumors. Therefore, the inhibition of cancer cells' metastasis is a highly significant issue. We previously demonstrated that particle (carbon ion beam) irradiation suppressed angiogenesis and metastasis. The purpose of this study is to clarify the mechanism of these suppressions.
Methods: Human lung adenocarcinoma cells (A549) and human umbilical vascular endothelial cells (HUVEC) were irradiated with 290MeV carbon particle at HIMAC and 4MV X-ray. After irradiation, WST assay, boyden chamber assay, matrigel invasion assay, and cell adhesion assay were performed. RNA was extracted and cDNA micro array and RT-PCR were carried out.
Results: The proliferation of A549 and HUVEC cells were down-regulated by carbon particle irradiation in dose-dependent manner, though sublethal X-irradiation enhanced proliferation of both two cell lines. The migration of each cells were also suppressed by only 0.25Gy carbon beam. The invasiveness of A549 was suppressed by 0.25Gy carbon beam 48 hours after irradiation. As far as cDNA expressions are concerned, anillin, actin-binding protein, was decreased in A549 cells by carbon particle. In HUVEC, some of angiogenesis related gene expressions were decreased, including neuropilin-1, CTGF, and ephrin-B2.
Conclusion: Particle (carbon ion beam) irradiation may suppress cancer cells' aggressiveness through down-regulation of the metastasis and angiogenesis related gene expression., The 5th Japan-US Cancer Therapy Symposium & The 5th S.Takahashi Memorial Joint Symposium},
 title = {Mechanism of metastasis and angiogenesis inhibition by particle irradiation},
 year = {2007}
}