{"created":"2023-05-15T14:45:11.809508+00:00","id":61763,"links":{},"metadata":{"_buckets":{"deposit":"aff01b56-65f4-4b9c-a86f-4f5b9f4620de"},"_deposit":{"created_by":1,"id":"61763","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"61763"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00061763","sets":["10:29"]},"author_link":["611370","611379","611369","611375","611376","611380","611378","611373","611372","611368","611371","611374","611377"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2006-09-08","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"我々はこれまでにヌード腫瘍(ヒト大細胞性肺癌由来：IA)を用いて光子線照射前後の同腫瘍のclonogenのgrowht kineticsの研究を行い、放射線抵抗性低酸素下分裂細胞の存在を明らかにしてきた。同細胞の再分裂増殖は、再発の源であり、分割照射時のrepairに続くrepopulationの本体と考えられた。当細胞は、P-53のエクソン6コドン192、P-16のエクソン2コドン101およびK-rasのエクソン1コドン12のpoint mutationを有している。また同腫瘍はトリプシン処理によらず物理的に単個細胞となる特性を有する。X線照射後に同腫瘍のclonogenのGrowth Kineticsを分析したところ、12Gy照射で１日目までの10倍以上の著しい生存率の回復（PLD repair）、３-５日以降に急速に生存率の上昇(accelerated repopulation)が起った。同腫瘍に対し炭素線照射後のclonogenの生存率の解析を行ったところ、Ｘ線と炭素線に３-５日以降のrepopulationは観られたが、１日目のPLD repair現象は炭素線照射では観られず、X線特有の現象であった。また、分子レベルでこの現象を解明するために遺伝子発現解析を行った。遺伝子発現解析はヌードマウス腫瘍における各線の非照射に対して照射直後、3時間、18時間後の遺伝子発現をDNA修復関連遺伝子に絞って半定量的PCR法にて行った。その結果、１０遺伝子について１８時間後で有意な発現変化が認められた。現在、HiCEP法を用いたゲノムワイドにスクリーニングを行っており炭素線照射後のPLD repairの抑制に関連する新規遺伝子の単離を試みている。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会 第４９回　札幌大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三枝, 公美子"}],"nameIdentifiers":[{"nameIdentifier":"611368","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻, 厚至"}],"nameIdentifiers":[{"nameIdentifier":"611369","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"相良, 雅史"}],"nameIdentifiers":[{"nameIdentifier":"611370","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀, 恒夫"}],"nameIdentifiers":[{"nameIdentifier":"611371","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"原田, 良信"}],"nameIdentifiers":[{"nameIdentifier":"611372","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"宮本, 忠昭"}],"nameIdentifiers":[{"nameIdentifier":"611373","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"その他"}],"nameIdentifiers":[{"nameIdentifier":"611374","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"三枝 公美子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"611375","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 厚至","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"611376","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"相良 雅史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"611377","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀 恒夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"611378","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"原田 良信","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"611379","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"宮本 忠昭","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"611380","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"「肺癌ヌード腫瘍における炭素線照射後のPLDrepairの抑制と関連遺伝子発現」","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"「肺癌ヌード腫瘍における炭素線照射後のPLDrepairの抑制と関連遺伝子発現」"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-09-12"},"publish_date":"2006-09-12","publish_status":"0","recid":"61763","relation_version_is_last":true,"title":["「肺癌ヌード腫瘍における炭素線照射後のPLDrepairの抑制と関連遺伝子発現」"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:38:34.808819+00:00"}