@misc{oai:repo.qst.go.jp:00061636,
 author = {Odaka, Kenichi and Uehara, Tomoya and Arano, Yasushi and Adachi, Seika and Tadokoro, Hiroyuki and Hasegawa, Hiroshi and Yoshida, Kyoko and Yoshida, Toshimichi and Hiroe, Michiaki and Irie, Toshiaki and Tanada, Shuji and Yoshida, Katsuya and 小高 謙一 and 上原 知也 and 荒野 泰 and 足立 清夏 and 田所 裕之 and 長谷川 洋 and 入江 俊章 and 棚田 修二 and 吉田 勝哉},
 month = {Nov},
 note = {Background: During tissue remodeling after acute myocardial infarction (MI), tenascin-C (TNC), one of extracellular matrix glycoproteins, loosens the strong adhesion of surviving cardiomyocytes at the border zone to reorganize the cell shape or arrangement.  To develop a new noninvasive diagnostic technique for the early tissue remodeling after acute MI, we tried to detect active region of tissue remodeling using radiolabeled anti-TNC antibody and single-photon emission computed tomography (SPECT).  Methods: 125I anti-TNC monoclonal antibody fragment was injected intravenously to 5 acute MI rats (D) at 5 days after operation, and 5 sham-operated rats (C), and the biodistribution of this radiotracer was measured.  Excised and sectioned hearts were stained with hematoxylin and eosin for histological analysis and were exposed to autoradiography.  Dual-isotope SPECT imaging of 111In anti-TNC antibody and 99mTc-MIBI was also performed to examine the feasibility of this tracer for in vivo imaging.  Results:  Average whole heart myocardial radioactivity was significantly higher in D than C (D, 0.19+/-0.02 %injected-dose/g; C, 0.12+/-0.00; p < 0.01).  Myocardial radioactivity in the infarcted area was also significantly higher in D than C (D, 0.20+/-0.02 %injected-dose/g; C, 0.11+/-0.01, p < 0.01)  By autoradiography, high radioactivities were observed in the periphery of the necrotic mass in the granulation tissue.  D-to-C ratio of the average autoradiographic intensity of myocardial tissue was 1.56.  The ratio of autoradiographic intensities of infarcted area to noninfarcted area was 1.77.  SPECT imaging demonstrated the regional myocardial uptake of 111In anti-TNC antibody, which was complementary to perfusion image.  In addition, tracer distribution in the infarcted area was relatively heterogeneous.  Conclusions: The present study indicated a high ability of the radiolabeled anti-TNC fragments to localize the infarcted region in the heart by ex-vivo and in-vivo imagings, suggesting its potential usefulness for the noninvasive detection of tissue remodeling., Scientific Session 2003},
 title = {Noninvasive Detection of Tissue Remodeling After Acute Myocardial Infarction in Rats by Radiolabeled Anti-Tenascin-C Antibody},
 year = {2003}
}