@misc{oai:repo.qst.go.jp:00061635,
 author = {Odaka, Kenichi and Toyozaki, Tetsuya and Uehara, Tomoya and Arano, Yasushi and Yoshida, Katsuya and Sato, Mikio and Hasegawa, Hiroshi and Tadokoro, Hiroyuki and Yoshida, Kyoko and Yoshida, Toshimichi and Hiroe, Michiaki and Irie, Toshiaki and Tanada, Shuji and Komuro, Issei and 小高 謙一 and 上原 知也 and 荒野 泰 and 吉田 勝哉 and 佐藤 幹生 and 長谷川 洋 and 田所 裕之 and 入江 俊章 and 棚田 修二},
 month = {Jun},
 note = {Background: The identification of inflammatory infiltrates in endomyocardial biopsy specimens is necessary for the definite diagnosis of myocarditis, despite an invasive method.  Expression of tenascin-C (TNC), one of the oligometric extracellular glycoproteins, is induced in myocardium during active stage of myocarditis.  Therefore, TNC may be a molecular marker for disease activity in myocarditis.  Methods: 111In-anti-TNC monoclonal antibody was injected intravenously to 10 experimental autoimmune myocarditis (EAM) rats and 10 control rats, and the biodistribution of this radiotracer was measured.  Excised and sectioned hearts were stained with hematoxylin and eosin for histological analysis and were exposed to autoradiography.  In vivo SPECT imaging was simultaneously performed with 111In-anti-TNC and 99mTc-Sestamibi.  The SPECT system consisted of a 3-headed gamma-camera equipped with 1.0-mm pinhole collimators.  Results: Average myocardial radioactivity was significantly higher in EAM rats than controls (2.39+/-0.17 %injected-dose/g vs. 0.32+/-0.03, p < 0.01).  By autoradiography, high radioactivities were observed in the regions identical with inflammation in EAM rats.  SPECT imaging in EAM rats clearly showed the focal myocardial uptake of 111In-anti-TNC, which is complementary to perfusion image.  Conclusions: The present study indicated a high ability of anti-TNC antibody to localize sites of inflammation in the heart by ex-vivo and in-vivo imaging.  Radiolabeled anti-TNC may be useful for the noninvasive diagnosis of myocarditis., 50th Annual Meeting of Society of Nuclear Medicine},
 title = {DUAL-ISOTOPE SPECT USING 111In ANTI-TENASCIN-C ANTIBODY AND 99mTc-SESTAMIBI TO ASSESS DISEASE ACTIVITY OF MYOCARDITIS IN RATS},
 year = {2003}
}