@misc{oai:repo.qst.go.jp:00061514,
 author = {三枝, 公美子 and 古野, 亜紀 and 石川, 顕一 and 石川, 敦子 and 大塚, 好美 and 川井, 聖子 and 野島, 久美恵 and 今井, 高志 and 三枝 公美子 and 古野 亜紀 and 石川 顕一 and 石川 敦子 and 荘司 好美 and 川井 聖子 and 野島 久美恵 and 今井 高志},
 month = {Apr},
 note = {It is recognized that carbon-ion beam kills cancer cells more efficiently than X-ray.  In this study we have compared cellular gene expression response after carbon-ion beam exposure with that after X-ray exposure.  Gene expression profiles of cultured neonatal human dermal fibroblasts (NHDF) at 0, 1, 3, 6, 12, 18, and 24hr after exposure to 0.1, 2 and 5Gy of X-ray or carbon-ion beam were obtained using 22K oligonucleotide microarray.  N-way ANOVA analysis of whole gene expression data sets selected 960 genes for carbon-ion beam and 977 genes for X-ray, respectively.  Interestingly, majority of these genes (91% for carbon-ion beam and 88% for X-ray, respectively) were down regulated.   The selected genes were further classified by their dose-dependence or time-dependence of gene expression change (fold change>1.5).    It was revealed that genes involved in cell proliferation had tendency to show time-dependent up regulation by carbon-ion beam.  Another N-way ANOVA analysis was performed to select 510 genes, and further selection was made to find 70 genes that showed radiation species-dependent gene expression change (fold change>1.25). These genes were then categorized by the K-Mean clustering method into 4 clusters.  Each cluster showed tendency to contain genes involved in cell cycle regulation, cell death, responses to stress and metabolisms, respectively., 重粒子線がん治療装置等共同利用研究成果発表会},
 title = {ヒト培養細胞を用いた重粒子線照射における遺伝子発現変化の検討},
 year = {2005}
}