{"created":"2023-05-15T14:44:38.372997+00:00","id":61025,"links":{},"metadata":{"_buckets":{"deposit":"a31796e8-cb7e-4c8f-b2e1-f55449e21ae9"},"_deposit":{"created_by":1,"id":"61025","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"61025"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00061025","sets":["10:29"]},"author_link":["604791","604808","604800","604802","604795","604793","604804","604801","604792","604797","604806","604805","604810","604809","604799","604790","604803","604794","604798","604796","604807"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2004-06-04","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"DNA double strand breaks (DSBs) are the most deleterious and lethal form of DNA damage. DSBs are rejoined or repaired by two major pathways in mammalian cells: i.e. homologous recombination (HR) and non-homologous end-joining (NHEJ). DNA-PK is a nuclear, serine/threonine protein kinase consisting of three subunits of DNA-PKcs, Ku70 and Ku80, and involved in the NHEJ, V(D)J recombination and modulation of transcription [1]. Cells lacking DNA-PKcs activity are highly sensitive to DSBs-inducing agents such as ionizing radiations [2]. Douglas et al [3] identified 4 phosphorylation sites in the DNA-PKcs protein: i.e. Thr-2609, Ser-2612, Thr-2638 and Thr-2647. These sites were autophosphorylated with own DNA-PKcs. Chan et al [4] demonstrated that autophosphorylation of DNA-PKcs was required for the rejoining of DSBs. \nRadiation sensitivities of 31 human esophageal squamous cell carcinoma (ESCC) cell lines were investigated with a colony-formation assay. There was a large variation in radiosensitivity among 31 cell lines. In particular, the radiation sensitivity of one cell line (KYSE190) was distinct from a cluster of radiation sensitivities of other 30 cell lines. In order to understand the mechanism behind this hypersensitivity, we investigated the expression of ATM and DNA-PKcs proteins with a western-blotting method and the phosphorylation of DNA-PKcs with a immunohistochemical staining methd. The phosphorylation of DNA-PKcs was not observed in KYSE190 cells. No mutation was detected in the four phosphorylation sites, but one base change in FAT domain of DNA-PKcs gene was observed. These data seem to indicate that the high radiosensitivity of KYSE 190 cells results from the defect in the autophosphorylation of the DNA-PKcs protein.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"The 5th Japan-France Workshop on Radiobiology and Medical Imaging","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ban, Sadayuki"}],"nameIdentifiers":[{"nameIdentifier":"604790","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"604791","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sagara, Masashi"}],"nameIdentifiers":[{"nameIdentifier":"604792","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Michikawa, Yuichi"}],"nameIdentifiers":[{"nameIdentifier":"604793","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Umetsu, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"604794","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakurai, Akio"}],"nameIdentifiers":[{"nameIdentifier":"604795","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishikawa, Ken-ichi"}],"nameIdentifiers":[{"nameIdentifier":"604796","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okabe, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"604797","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimada, Yutaka"}],"nameIdentifiers":[{"nameIdentifier":"604798","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Inazawa, Jouji"}],"nameIdentifiers":[{"nameIdentifier":"604799","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"604800","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"604801","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伴 貞幸","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"604802","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"604803","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"相良 雅史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"604804","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"道川 祐市","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"604805","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"梅津 篤","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"604806","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"櫻井 亜希子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"604807","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石川 顕一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"604808","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡部 篤史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"604809","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 高志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"604810","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"A deficiency in the phosphorylation of DNA-PKcs in a radiosensitive human ESCC cell line","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A deficiency in the phosphorylation of DNA-PKcs in a radiosensitive human ESCC cell line"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2005-07-07"},"publish_date":"2005-07-07","publish_status":"0","recid":"61025","relation_version_is_last":true,"title":["A deficiency in the phosphorylation of DNA-PKcs in a radiosensitive human ESCC cell line"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:47:04.475915+00:00"}