{"created":"2023-05-15T14:43:58.493846+00:00","id":60138,"links":{},"metadata":{"_buckets":{"deposit":"4d2dede4-c7d4-40a3-b6b0-2ab781c2cd87"},"_deposit":{"created_by":1,"id":"60138","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"60138"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00060138","sets":["10:29"]},"author_link":["597982","597985","597983","597986","597987","597984"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2002-10-03","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"サイクリンキナーゼ阻害作用をもつp21は、DNA 損傷に応答し、G1/S進行抑制、Ｓ期遅延、G2/M 進行遅延を引き起こす。今回、DNA 損傷後のS-G2/M進行抑制におけるp21のCyclinA 結合の制御を知るため、Cy motifあるいはCdk2リン酸化部位を順次欠落させたHisタグ変異p21を真核細胞に発現させ、1-10Gyのガンマ線照射24h後、非変性条件でHisタグp21をNi精製して、結合しているCyclinA、Cdk2およびCyclinE量の変動を解析した。 全長 (1-164) , 1-157(ΔNLS)、1-147(ΔCy2)、1-128(Δ130SP)、1-96(Δ98SP)、21-164(ΔCy1)、21-147(ΔCy1,Cy2) の何れのp21においてもガンマ線量に依存してCyclinA 結合が増加した。一方、結合しているCdk2、およびCyclinE量の変動は顕著でなかった。p21のCyclinA 結合はCy motifを要求せず、他の結合制御機構の存在が示唆された。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第61回日本癌学会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"福地, 邦彦"}],"nameIdentifiers":[{"nameIdentifier":"597982","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"市村, 幸子"}],"nameIdentifiers":[{"nameIdentifier":"597983","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"巽, 紘一"}],"nameIdentifiers":[{"nameIdentifier":"597984","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"五味, 邦英"}],"nameIdentifiers":[{"nameIdentifier":"597985","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"沼田 幸子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"597986","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"巽 紘一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"597987","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"DNA傷害後のCdkインヒビターp21とCyclinA結合にはCy motifを要求しない","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"DNA傷害後のCdkインヒビターp21とCyclinA結合にはCy motifを要求しない"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2003-11-17"},"publish_date":"2003-11-17","publish_status":"0","recid":"60138","relation_version_is_last":true,"title":["DNA傷害後のCdkインヒビターp21とCyclinA結合にはCy motifを要求しない"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:57:05.131439+00:00"}