@misc{oai:repo.qst.go.jp:00059651,
 author = {Takeshita, Keizo and Cuiping, Chi and Hirata, Hiroshi and Ono, Mitsuhiro and Suzaki, Hitoshi and Anzai, Kazunori and Ozawa, Toshihiko and 竹下 啓蔵 and 池 翠萍 and 小野 光弘 and 安西 和紀 and 小澤 俊彦},
 month = {Apr},
 note = {Exposure to ultraviolet (UV) light leads to various injuries such as sunburn, inflammation, aging, cancer, etc.  There have been little reports about in vivo evidence of generation of free radicals including oxygen radicals during exposure to UV light, although free radicals may be involved in those injuries.  In this study, induction of radical reaction was examined in skin of living mouse under UV light by using in vivo ESR spectroscopy with a nitroxyl radical, 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-N-oxyl (carbamoyl-PROXYL), as a redox probe.  An aqueous solution of carbamoyl-PROXYL was injected intravenously to an anesthetized mouse, and L-band ESR spectra of the probe were recorded at the dorsal region of hairless mice with a surface-coil-type resonator.  ESR signal of carbamoyl-PROXYL increased up to a few minutes after the injection and then decreased.  The signal intensity for mouse whose skin was removed and then returned was about two-third of the intensity for non-treated mouse, indicating that the signal of probe in skin should be about one-third of signal observed in non-treated mouse.  The rate of the signal decay increased by irradiation with UV light (UV-A+B).  The increase was statistically significant.  The increase of signal decay rate was not observed in mouse with removed, and then returned skin, indicating that the enhancement of signal decay should occur in skin.  The increase of signal decay rate was suppressed by pre-administration of a spin trapping reagent, N-t-butyl-a-phenylnitrone (PBN), while PBN did not change the decay rate for non-irradiated mouse.  There was no relationship between the signal decay rate and physical parameters (blood velocity and blood mass in skin and body surface temperature).  These observations suggest that the measurement of radical generation under UV light is possible by using in vivo ESR spectroscopy and a nitroxyl redox probe., The 10th International Workshop on Bio-Medical ESR Spectroscopy and Imaging},
 title = {Topical Measurement of radical Generation in Mouse Skin under Ultraviolet Light: In vivo ESR Study with a Surface Coil-Type Resonator},
 year = {2003}
}