@misc{oai:repo.qst.go.jp:00059383,
 author = {Matsumoto, Hideki and Hayashi, Saxhiko and Hui, Jin Zhao and Hatashita, Masanori and Kano, Eiichi and Shao, Chunlin and Furusawa, Yoshiya and 松本 英樹 and Ｓｈａｏ Ｃｈｕｎｌｉｎ and 古澤 佳也},
 month = {Aug},
 note = {PURPOSE: To investigate whether nitric oxide excreted from irradiated cells with accelerated carbon ion beams modulates cellular radiosensitivity against the accelerated carbon ion beams in human glioblastoma cells bearing either wild-type p53 or mutant p53. 
MATERIALS AND METHODS: Western blot analysis of inducible nitric oxide synthase, hsp72 and p53, the concentration assay of nitrite in medium and cell survival assay after irradiation with accelerated carbon ion beams were performed. 
RESULTS: The accumulation of inducible nitric oxide synthase was caused by accelerated carbon ion beam irradiation of mutant p53 cells but not of wild-type p53 cells. The accumulation of hsp70 and p53 was observed in wild-type p53 cells after exposure to the conditioned medium from the irradiated mutant p53 cells with accelerated carbon ion beams, and the accumulation was abolished by the addition of an inhibitor for inducible nitric oxide synthase to the medium. The radiosensitivity of wild-type p53 cells was reduced in the conditioned medium from the irradiated mutant p53 cells with accelerated carbon ion beams compared with conventional fresh growth medium, and the reduction of radiosensitivity was abolished by the addition of a specific inducible nitric oxide synthase inhibitor to the conditioned medium. 
CONCLUSIONS: Nitric oxide excreted from the irradiated donor cells with accelerated carbon ion beams could modulate radiosensitivity of recipient cells against the accelerated carbon ions. These findings indicate the importance of an intercellular signal transduction pathway initiated by nitric oxide in the cellular response to accelerated heavy ions., The First International Symposium on Ion Beams: Biological Effects and Molecular Mechanisms},
 title = {Nitric Oxide is an Initiator of the Bystander Effect Induced by Heavy-ion Beams},
 year = {2002}
}