{"created":"2023-05-15T14:43:19.173985+00:00","id":59339,"links":{},"metadata":{"_buckets":{"deposit":"6dda612f-fb2c-4ac8-a439-15fc30429f71"},"_deposit":{"created_by":1,"id":"59339","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"59339"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00059339","sets":["10:29"]},"author_link":["590634","590630","590627","590639","590629","590628","590640","590646","590625","590642","590632","590647","590637","590633","590626","590643","590636","590644","590624","590631","590638","590641","590635","590645"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2002-09-20","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"重粒子線が細胞核を通過すると、そのトラックに沿ってDNA切断が局在する。この局在したDNA切断を可視化できれば、重粒子線の生物効果誘発機構の解明に有効な手段を提供する。そこで、DNA主鎖切断の3’末端OH基に、TdT（terminal deoxyribotransferase）を用いて、BrdU（bromodeoxyuridine）を結合させ、FITC標識anti-BrdU抗体で免疫蛍光染色し、可視化を試みたので報告する。\nスライドグラスに接着させたCV-1細胞（アフリカミドリザル腎由来細胞）に、HIMAC中エネルギー照射室で得られる鉄イオン（水中LET=4,690 keV/_m）を照射した。細胞を固定後、上記の方法でDNA切断端を標識した。核の対比染色には、PI (propidium iodide)を用いた。フルエンス（particles/m2）は、固体飛跡検出用プラスチックCR39で計測し、細胞の平均核面積（3.5×10-10 _m2）あたりに照射されたイオン数を求めた。試料は、共焦点顕微鏡で観察した。\n非照射の細胞には、明瞭なFITC蛍光のフォーカスは認められなかったが、照射した細胞の核内には明瞭な蛍光のフォーカスが観察できた。このフォーカスの数は、細胞核あたり照射したイオン数に比例して増加した：イオン数17、32、45に対応して、フォーカス数13、27、45。この比例関係は、今回可視化したフォーカスがイオントラックに沿って誘発されたDNA主鎖切断を可視化していることを示す。今後、今回可視化したフォーカスの形状を計測することによって、イオントラック構造に関する情報を得たり、各種DNA修復酵素のフォーカスとの、空間的時間的な相関関係を調べることによって、in vivo DNA修復に関する情報を取得したい。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会第４５回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only 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仲宏"}],"nameIdentifiers":[{"nameIdentifier":"590630","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐藤, 幸夫"}],"nameIdentifiers":[{"nameIdentifier":"590631","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤, 佳也"}],"nameIdentifiers":[{"nameIdentifier":"590632","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石澤, 紗智"}],"nameIdentifiers":[{"nameIdentifier":"590633","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山浦, 晋"}],"nameIdentifiers":[{"nameIdentifier":"590634","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"檜枝, 光太郎"}],"nameIdentifiers":[{"nameIdentifier":"590635","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"竹安 明弘","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"590636","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小西 輝昭","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"590637","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"冨田 雅典","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"590638","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中島 宏","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"590639","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡庭 達也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"590640","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本 健一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"590641","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安田 仲宏","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"590642","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐藤 幸夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"590643","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤 佳也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"590644","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石澤 紗智","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"590645","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山浦 晋","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"590646","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"檜枝 光太郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"590647","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"細胞核内重粒子線トラックの可視化(1)　BrdUによるDNA切断端の標識","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"細胞核内重粒子線トラックの可視化(1)　BrdUによるDNA切断端の標識"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2002-07-04"},"publish_date":"2002-07-04","publish_status":"0","recid":"59339","relation_version_is_last":true,"title":["細胞核内重粒子線トラックの可視化(1)　BrdUによるDNA切断端の標識"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T22:06:02.704322+00:00"}