@article{oai:repo.qst.go.jp:00056019, author = {Kato, Yuichi and Ho, Shih-Hsin and Nakanishi, Akihiko and Ogino, Chiaki and Hasunuma, Tomohisa and 佐藤, 勝也 and 大野, 豊 and Kondo, Akihiko and 佐藤 勝也 and 大野 豊}, journal = {QST Takasaki Annual Report 2015}, month = {Mar}, note = {Microalgae are expected to be excellent producers of a renewable energy resource that does not compete with the current agricultural properties. We have developed strategies for lipid production using an oleaginous green microalga Chlamydomonas sp. JSC4 isolated from the Taiwanese sea coast. We previously found that JSC4 accumulates lipid in the presence of sea salt (SS), but also easily infected opportunistically by a parasitic Chytridiomycota under salinity stress when cultivated outdoor. To solve this problem, this study aims to obtain salt-resistant strains by mutation breeding using the carbon ion beams and habituation to the high-salinity condition. As a result, wild type JSC4 cells can grow under 3% SS but not under 7% SS. We bred a salt-resistant strain (KHI) from JSC4 by irradiating it with the carbon ion beams and habituating to salinity environment.}, pages = {139--139}, title = {Mutation Breeding of Microalga Strains Resistant to Hyper-salinity Stress}, year = {2017} }