{"created":"2023-05-15T14:40:46.582435+00:00","id":55924,"links":{},"metadata":{"_buckets":{"deposit":"e45cd5d9-8387-43d6-add8-a1211f4148d6"},"_deposit":{"created_by":1,"id":"55924","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"55924"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00055924","sets":["7"]},"author_link":["865450","865445","865443","865444","865441","865451","865442","865448","865453","865446","865452","865447","865449"],"item_10004_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"23","bibliographicPageStart":"14","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"放射線医学総合研究所　技術報告書（研究基盤技術）"}]}]},"item_10004_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Prepubertal females have often been preferred for procuring oocytes in laboratory and domestic animals. However, oocytes from prepubertal animals are less competent to develop into fetuses than those from mature animals. In this study, we compared the developmental competence of oocytes from animals of various ages (3–6, 8, and 10 weeks old) to examine the efficiency of using prepubertal animals as oocyte donors for in vitro animal production, and to elucidate the age at which animals acquire full developmental competence. Oocytes from superovulated C57BL/6J females at various ages were collected and fertilized in vitro, and subsequent development was examined by in vitro culture and embryo transfer. Total numbers of ovulated oocytes reduced with age and a significantly higher percentage of morphologically normal oocytes were obtained from 3-week-olds animals (99%) than from animals of other ages (£86%, P < 0.05). No significant differences were observed in percentages of fertilization (³97%). Development to 2-cell and blastocyst stages in vitro, nuclear numbers in blastocysts and fetus development after embryo transfer on day 19 of gestation were lowest in embryos from 3-week-old animals than those from older animals. These results indicate that the quality of oocytes from juvenile mice was different and that oocytes acquired full developmental competence from 4-week-old or later.\nKeywords: Juvenile mice, in vitro fertilization, in vitro development, fetus development","subitem_description_type":"Abstract"}]},"item_10004_relation_10":{"attribute_name":"ISBN","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"978-4-938987-89-3","subitem_relation_type_select":"ISBN"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鬼頭, 靖司"}],"nameIdentifiers":[{"nameIdentifier":"865441","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"太田, 有紀"}],"nameIdentifiers":[{"nameIdentifier":"865442","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"和田, 彩子"}],"nameIdentifiers":[{"nameIdentifier":"865443","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伊林, 恵美"}],"nameIdentifiers":[{"nameIdentifier":"865444","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"林, 真美"}],"nameIdentifiers":[{"nameIdentifier":"865445","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"矢野, 浩子"}],"nameIdentifiers":[{"nameIdentifier":"865446","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塚本, 智史"}],"nameIdentifiers":[{"nameIdentifier":"865447","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kito, Seiji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"865448","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wada, Ayako","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"865449","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ibayashi, Megumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"865450","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hayashi, Mami","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"865451","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yano, Hiroko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"865452","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsukamoto, Satoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"865453","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"性成熟前から性成熟の期間におけるC57BL/6J卵子の受精・発生能の比較","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"性成熟前から性成熟の期間におけるC57BL/6J卵子の受精・発生能の比較"}]},"item_type_id":"10004","owner":"1","path":["7"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-12-10"},"publish_date":"2014-12-10","publish_status":"0","recid":"55924","relation_version_is_last":true,"title":["性成熟前から性成熟の期間におけるC57BL/6J卵子の受精・発生能の比較"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:58:29.185153+00:00"}