@article{oai:repo.qst.go.jp:00055912,
 author = {小林, 亜利紗 and 山縣, 徳嗣 and 須藤, 和美 and ウォーレン, 圭子 and 小西, 輝昭 and 小林 亜利紗 and 山縣 徳嗣 and 森口 和美 and ウォーレン 圭子 and 小西 輝昭},
 journal = {放射線医学総合研究所　技術報告書（研究基盤技術）},
 month = {Mar},
 note = {The micro-beam irradiation technique has become a powerful method in radiation biological studies. In brief, the SPICE-NIRS microbeam can target cell nuclei, cytoplasm, or both automatically with a defined number of protons. For example, a cell sample with 3000 cells per dish can be irradiated within 15 minutes including all the necessary procedures such as image capturing and cell recognition analysis. However, there are several limitations in both machinery components such as microscopes, beam lines, sample stages, biological aspects, cell lines, and cell dishes and in fluorescence staining, which is necessary for targeting; these limitations needs to be overcome for accomplishing this performance.
 In this study, we present a protocol for constructing the cell dish that was specially designed for the SPICE-NIRS microbeam. The dish consists of four components as follows: (1) ring, (2) base (both assists to sandwich thin film and screws), (3) thin film (for attachment of the cells), and (4) screws (for fixing the other components). The dishes are not disposable and may be sterilized and reused for each beam time by researchers. This protocol is written in both Japanese and English for domestic and foreign researchers.},
 pages = {24--33},
 title = {放医研マイクロビーム装置SPICE専用細胞皿の作成方法},
 volume = {8},
 year = {2014}
}