@article{oai:repo.qst.go.jp:00055896,
 author = {松本, 孔貴 and 鵜澤, 玲子 and 山下, 慶 and 李, 惠子 and 平山, 亮一 and 幸田, 華奈 and 尾崎, 匡邦 and 陳, 剣 and 安藤, 興一 and 増永, 慎一郎 and 古澤, 佳也 and 松本 孔貴 and 鵜澤 玲子 and 山下 慶 and 李 惠子 and 平山 亮一 and 幸田 華奈 and 尾崎 匡邦 and 陳 剣 and 安藤 興一 and 古澤 佳也},
 journal = {2012 Annual Report of the Research Project with Heavy Ions at NIRS-HIMAC},
 month = {Aug},
 note = {The fractionated irradiation is standard protocol
for radiotherapy. There are big differences between single and fractionation, for example the DNA repair and reoxygenation of hypoxic region between the fractionation. It is necessary to get the results using fractionated irradiation to know the effects in clinical. The purpose of this study is to examine the effects of fractionated irradiation for metastasis and to evaluate the relationship of hypoxic cells with tumor metastatic potentials. A mouse osteosarcoma, LM8 cells were inoculated in mice, and the tumors were irradiated with C-ions at the entrance and center of SOBP position. Tumor cell survival in vivo and metastatic fraction were examined by in vivo-in vitro assay and spontaneous lung metastasis model, respectively. After fractionated irradiation, tumor cell survival in vivo and the lung metastasis also showed the tendency of increase depend on the fraction number, and these effects were also more remarkable by X-rays than C-ions. The effect of 1 % hypoxic conditions to tumor cells survival and cell migration ability using colony formation assay and Boyden chamber assay, respectively. Continuous 1 % hypoxic condition (6h to 12h) enhanced cell migration ability compared with the ability of cells cultured in oxic condition. LM8 inoculated tumors showed reoxygenation after C-ion at 12 to 18 h in the tumor growth suppression. However, the number of lung metastasis did not show the significant difference depending on the reoxygenation.},
 pages = {159--160},
 title = {分割照射の転移への影響},
 year = {2013}
}