@article{oai:repo.qst.go.jp:00055803, author = {前田, 武 and 高野, 裕之 and 小澤ブル, 美絵 and 中村, 悦子 and 下川, 卓志 and 小西, 輝昭 and 前田 武 and 高野 裕之 and 小澤ブル 美絵 and 中村 悦子 and 下川 卓志 and 小西 輝昭}, issue = {NIRS-M-253}, journal = {放射線医学総合研究所技術報告書}, month = {Nov}, note = {FACSAria, manufactured by Becton Dickinson (BD Biosciences, San Jose, USA), is a high-speed cell sorter for counting and sorting fluorescently labeled cells. This instrument was purchased in 2005 for use in radiobiological studies conducted at NIRS and was managed as "Joint Use Facilities and Equipments" for collaborative research. Compared to previous commercial instruments, this version of the instrument was designed to be easier to use. However, multiple steps and careful guidance were still necessary in order to adhere to the high specifications for the instrument. Therefore, in 2006, an unofficial team called "Team FACs" was formed to develop knowledge on using FACSAria so that this team could offer technical assistance and biological and mechanical advice for using this instrument. Along with this team, we prepared an instruction manual for sorting cells into 2 tubes, which is called 2-way sorting. The latest version of this instruction manual is now complete, and we hope that this manual would provide technical guidance to users of FACSAria.}, pages = {35--80}, title = {Instruction Manual for 2-Way Sorting Using BD FACSAria}, volume = {2012}, year = {2012} }