{"created":"2023-05-15T14:40:36.783799+00:00","id":55721,"links":{},"metadata":{"_buckets":{"deposit":"6bd26f0b-f85e-49e8-99d1-d1df849f5c79"},"_deposit":{"created_by":1,"id":"55721","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"55721"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00055721","sets":["7"]},"author_link":["570169","570165","570166","570167","570168","570170","570162","570163","570164"],"item_10004_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"NIRS-M-237","bibliographicPageEnd":"53","bibliographicPageStart":"45","bibliographicVolumeNumber":"2010","bibliographic_titles":[{"bibliographic_title":"放射線医学総合研究所技術報告書"}]}]},"item_10004_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"放射線医学総合研究所(以下「放医研」という)には、共同実験機器 として「細胞分取装置」と言う名称で日本BD(Becton, Dickinson and Company)社のFACSAriaが導入されている。FACSAria はフローサイトメトリー(Flow Cytometry)  の一種で、血球細胞、動物細胞、植物細胞、酵母、バクテリア、プランクトン、核などの0.5 µm〜100 µm程度の大きさの単離状態の細胞浮遊液のサンプル1つ1つにレーザー光を当てて、出てきた散乱光や蛍光を検出し、それらの光を電気信号に変えて検出し測定する装置である。\n今までは、本装置に対する精度を維持するためのメンテナンス等についてと、最も特徴的であるソーティング精度について検証を重ね報告をしてきた。\n今回は、搭載している3本のレーザー(青色波長:488nm　半導体レーザー、赤色波長:633nm　HeNeレーザー、紫色波長:407nm 半導体レーザー)の内、407nm半導体レーザーを用いてHoechst染色した細胞の検出の可否について検討を行った。","subitem_description_type":"Abstract"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"前田, 武"}],"nameIdentifiers":[{"nameIdentifier":"570162","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小西, 輝昭"}],"nameIdentifiers":[{"nameIdentifier":"570163","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見, 尚子"}],"nameIdentifiers":[{"nameIdentifier":"570164","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高野, 裕之"}],"nameIdentifiers":[{"nameIdentifier":"570165","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"児玉, 久美子"}],"nameIdentifiers":[{"nameIdentifier":"570166","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"前田 武","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"570167","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小西 輝昭","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"570168","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見 尚子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"570169","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高野 裕之","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"570170","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"FACSAriaによるHoechst蛍光試薬を用いたソーティングの検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"FACSAriaによるHoechst蛍光試薬を用いたソーティングの検討"}]},"item_type_id":"10004","owner":"1","path":["7"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-02-25"},"publish_date":"2011-02-25","publish_status":"0","recid":"55721","relation_version_is_last":true,"title":["FACSAriaによるHoechst蛍光試薬を用いたソーティングの検討"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T22:46:10.839681+00:00"}