@article{oai:repo.qst.go.jp:00055690,
 author = {松本, 孔貴 and 平山, 亮一 and 崔, 星 and 古澤, 佳也 and 安藤, 興一 and 鵜澤, 玲子 and 高瀬, 信宏 and 小池, 幸子 and 岡安, 隆一 and 松本 孔貴 and 平山 亮一 and 崔 星 and 古澤 佳也 and 安藤 興一 and 鵜澤 玲子 and 高瀬 信宏 and 小池 幸子 and 岡安 隆一},
 journal = {放射線医学総合研究所重粒子線がん治療装置等共同利用研究報告書},
 month = {Jun},
 note = {The aim of this study is to clarify the time and dose dependency of cell cycle in tumor cells exposed to Carbon-ions (C-ions) and X-rays. Five human malignant melanoma cell lines, 92-1, C32TG, HMV-I, HMV-II and MeWo were used. Cells were exposed to X-rays or 290 MeV/u C-ions at the center of SOBP. The cell survival after irradiation was obtained using a colony formation method. The 10% survival dose (D10) were estimated from cell survival curves as iso-effect dose, and used for the time dependency analysis. The same physical dose of C-ions and X-rays was used for dose dependency analysis. After irradiation, the cells were trypsinized and re-seeded to dishes in appropriate concentration, and cultured in CO2 incubator. Cells were collected at adequate time point (6-204h after re-seeding), dyed with PI, fixed with Ethanol, and observed by the flow cytometry technique. After irradiation, the percentage of cells in G2/M phase was immediately increased, and the G2/M showed the peak between 12 to 18h in all cell lines. Additionally, it was clarify the HMV-I cells exposed to C-ions still existed in the G2/M phase at a high rate compared to X-rays and non-irradiated cells for long time (-200h). On the other hand, the fraction of cells arrested in G2/M was increased depending on the physical dose, and C-ions induced G2 arrest effectively more than X-rays using same physical dose. It was suggested that the transient prolonged G2/M arrest was specific phenomenon induced by carbon-ion beams.},
 title = {悪性腫瘍由来細胞における重粒子線の生物効果と細胞周期（20B486)},
 year = {2009}
}