@article{oai:repo.qst.go.jp:00055075,
 author = {大西, 武雄 and 大西, 健 and 高橋, 昭久 and 玉本, 哲朗 and 浅川, 勇雄 and 古澤, 佳也 and 大西 武雄 and 大西 健 and 高橋 昭久 and 玉本 哲郎 and 浅川 勇雄 and 古澤 佳也},
 journal = {放射線医学総合研究所重粒子線がん治療装置等共同利用研究報告書},
 month = {Apr},
 note = {To examine whether combination therapies of radiation and hyperthermia is p53-dependent, we analyzed tumor growth inhibition, accumulation of apoptosis-related proteins and incidence of apoptosis by using transplanted human tongue carcinomas with different p53 genes into nude mice. Human tongue squamous cell carcinoma cells (SAS) transfected with mutant p53 gene (SAS/mp53) and the neo gene (SAS/neo) and were transplanted by a trocar into the thigh of each nude mouse. When the mean diameter of tumors reached to 5-6 mm, heat treatment was performed. Tumor regrowth was evaluated when the RW reached 5-fold against the control group. In addition, apoptosis-related proteins and apoptotic
cells in the sections were measured by staining using immunohistochemical methods. SAS/mp53 tumors showed more heat-resistant than SAS/neo tumors.
Synergistic enhancement of tumor regrowth delay was observed by combination of X-rays at 2 Gy or C-beams at 1 Gy with hyperthermia (42degree-C, 20 min) in SAS/neo tumors, but not SAS/mp53 tumors. The incidence of apoptosis by the combined with hyperthermia and X-ray or C-beam treatments was very high in SAS/neo tumors compared with that in SAS/mp53 tumors. These results indicate that the thermal-enhancement of tumor growth inhibition of a transplanted human tongue carcinomas was p53-dependent, and might be deeply correlated with the induction of apoptosis.},
 pages = {57--58},
 title = {癌関連遺伝子からみた重粒子線治療の基礎的研究},
 volume = {2001},
 year = {2001}
}