@inproceedings{oai:repo.qst.go.jp:00054790,
 author = {Nakanishi, Ikuo and Ozawa, Toshihiko and Matsumoto, Kenichiro and 中西 郁夫 and 小澤 俊彦 and 松本 謙一郎},
 book = {Free Radical Biology and Medicine},
 issue = {Supplement},
 month = {Nov},
 note = {2,2-Diphenyl-1-picrylhydrazyl radical (DPPH●) is a relatively stable radical and frequently used as a reactivity model of reactive oxygen species. However, its insolubility in water precludes the investigation of the redox behavior of DPPH● in aqueous media, especially in concentrated buffer solutions. We have recently reported that DPPH● can be successfully solubilized in water by β-cyclodextrin (β-CD). The as-obtained β-CD-solubilized DPPH● (DPPH●/β-CD) was demonstrated to be a powerful tool for evaluating the antioxidative activity of water-soluble antioxidants, such as ascorbic acid, Trolox, and caffeic acid, in concentrated buffer solutions. In this study, the redox behavior of DPPH●/β-CD was investigated by cyclic voltammetry. A well-defined reversible redox wave was observed for one-electron reduction of DPPH●/β-CD in phosphate buffer (0.1 M, pH 7.4). On the other hand, one-electron oxidation of DPPH●/β-CD showed a quasi-reversible redox wave, which suggests that DPPH cations (DPPH+) generated by the electrochemical one-electron oxidation may undergo a follow-up chemical reaction, such as an addition of a water molecule.},
 pages = {S27--S28},
 publisher = {Elsevier},
 title = {Redox Behavior of 2,2-Diphenyl-1-Picrylhydrazyl Radical (DPPH∙) Solubilized in Water by β-Cyclodextrin},
 volume = {100},
 year = {2016}
}