@inproceedings{oai:repo.qst.go.jp:00054488, author = {Wing, Yan Choi Viann and Yuen, Ping Ng Candy and Kobayashi, Alisa and Konishi, Teruaki and Oikawa, Masakazu and Han, Cheng Shuk and Kwan, Ngok Yu Peter and 小林 亜利紗 and 小西 輝昭 and 及川 将一}, book = {Journal of Radiation Research}, issue = {S1}, month = {Feb}, note = {The microbeam irradiation system (single-particle irradiation system to cell, acronym as SPICE) at the National Institute of Radiological Sciences (NIRS), Japan, was employed to irradiate dechorionated embryos of the zebrafish, Danio rerio, at 5 h post-fertilization (hpf) by protons each having an energy of ∼3.4 MeV. Either 1 or 10 positions on the cells were irradiated with different number of protons. The levels of apoptosis in zebrafish embryos at 25 hpf were quantified through terminal dUTP transferase-mediated nick end-labeling assay. Triphasic dose–esponses were obtained (Fig. 1), including (i) a subhormetic zone with an increase in apoptotic signals for a small number of irradiated protons per position, (ii) a hormetic zone with a reduction in the apoptotic signals below the spontaneous level for a larger number of irradiated protons per position and (iii) a toxic zone with an increase in apoptotic signals again if the number of irradiated protons per position was further increased.}, publisher = {Oxford Journals, Oxford University Press}, title = {Response of 5 hpf zebrafish embryos to low-dose microbeam protons}, volume = {55}, year = {2014} }