{"created":"2023-05-15T14:39:06.193749+00:00","id":53716,"links":{},"metadata":{"_buckets":{"deposit":"738fd770-8240-4c6d-8f3e-f26c7dd4621f"},"_deposit":{"created_by":1,"id":"53716","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"53716"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00053716","sets":["2"]},"author_link":["548175","548171","548182","548166","548180","548173","548184","548185","548172","548174","548177","548183","548167","548178","548169","548179","548170","548176","548168","548181"],"item_10003_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-06","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"66","bibliographicPageStart":"62","bibliographic_titles":[{"bibliographic_title":"Proceedings of the Japan-France Workshop on Radiobiology and Isotopic Imaging"}]}]},"item_10003_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"DNA double strand breaks (DSBs) are the most deleterious and lethal form of DNA damage. DSBs are rejoined or repaired by two major pathways in mammalian cells: i.e. homologous recombination (HR) and non-homologous end-joining (NHEJ). DNA-PK is a nuclear, serine/threonine protein kinase consisting of three subunits of DNA-PKcs, Ku70 and Ku80, and involved in the NHEJ, V(D)J recombination and modulation of transcription [1]. Cells lacking DNA-PKcs activity are highly sensitive to DSBs-inducing agents such as ionizing radiations [2]. Douglas et al [3] identified 4 phosphorylation sites in the DNA-PKcs protein: i.e. Thr-2609, Ser-2612, Thr-2638 and Thr-2647. These sites were autophosphorylated with own DNA-PKcs. Chan et al [4] demonstrated that autophosphorylation of DNA-PKcs was required for the rejoining of DSBs. \nRadiation sensitivities of 31 human esophageal squamous cell carcinoma (ESCC) cell lines were investigated with a colony-formation assay. There was a large variation in radiosensitivity among 31 cell lines. In particular, the radiation sensitivity of one cell line (KYSE190) was distinct from a cluster of radiation sensitivities of other 30 cell lines. In order to understand the mechanism behind this hypersensitivity, we investigated the expression of ATM and DNA-PKcs proteins with a western-blotting method and the phosphorylation of DNA-PKcs with a immunohistochemical staining methd. The phosphorylation of DNA-PKcs was not observed in KYSE190 cells. No mutation was detected in the four phosphorylation sites, but one base change in FAT domain of DNA-PKcs gene was observed. These data seem to indicate that the high radiosensitivity of KYSE 190 cells results from the defect in the autophosphorylation of the DNA-PKcs protein.","subitem_description_type":"Abstract"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ban, Sadayuki"}],"nameIdentifiers":[{"nameIdentifier":"548166","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"548167","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sagara, Masashi"}],"nameIdentifiers":[{"nameIdentifier":"548168","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Michikawa, Yuichi"}],"nameIdentifiers":[{"nameIdentifier":"548169","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Umetsu, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"548170","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakurai, Akio"}],"nameIdentifiers":[{"nameIdentifier":"548171","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishikawa, Ken-ichi"}],"nameIdentifiers":[{"nameIdentifier":"548172","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okabe, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"548173","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimada, Yutaka"}],"nameIdentifiers":[{"nameIdentifier":"548174","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Inazawa, Jouji"}],"nameIdentifiers":[{"nameIdentifier":"548175","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"548176","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伴 貞幸","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"548177","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"548178","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"相良 雅史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"548179","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"道川 祐市","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"548180","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"梅津 篤","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"548181","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"櫻井 亜希子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"548182","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石川 顕一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"548183","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡部 篤史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"548184","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 高志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"548185","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference paper","resourceuri":"http://purl.org/coar/resource_type/c_5794"}]},"item_title":"A deficiency in the phosphorylation of DNA-PKcs in a radiosensitive human ESCC cell line","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A deficiency in the phosphorylation of DNA-PKcs in a radiosensitive human ESCC cell line"}]},"item_type_id":"10003","owner":"1","path":["2"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-06-13"},"publish_date":"2006-06-13","publish_status":"0","recid":"53716","relation_version_is_last":true,"title":["A deficiency in the phosphorylation of DNA-PKcs in a radiosensitive human ESCC cell line"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:09:26.724148+00:00"}