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Roles of protein kinase C in radiation-induced apoptosis signaling pathways in murine thymic lymphoma cells (3SBH5 cells)
https://repo.qst.go.jp/records/53126
https://repo.qst.go.jp/records/53126c1f7add2-3bb2-4231-9828-94cd504373a1
| Item type | 会議発表論文 / Conference Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-10-20 | |||||
| タイトル | ||||||
| タイトル | Roles of protein kinase C in radiation-induced apoptosis signaling pathways in murine thymic lymphoma cells (3SBH5 cells) | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_5794 | |||||
| 資源タイプ | conference paper | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakajima, Tetsuo
× Nakajima, Tetsuo× Yukawa, Osami× Ohyama, Harumi× Bing, Wang× Hayata, Isamu× Inaba, Hiroko× 中島 徹夫× 湯川 修身× 大山 ハルミ× 王 冰× 早田 勇× 稲葉 浩子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Murine thymic lymphoma cells and 3SBH5 are quite sensitive to X-rays and undergo apoptosis shortly after X-irradiation. Phorbol 12-myristate 13-acetate (PMA), an activator of protein kinase C (PKC), blocked the radiation-induced apoptosis in the 3SBH5 cells. On the other hand, chelerythrine, a PKC inhibitor, enhanced the radiation-induced apoptosis. These results suggest that PKC plays a key role in the regulation of radiation-induced apoptosis in 3SBH5 cells. Irradiation alone had no effect on the distribution of PKC subtypes (alpha, betaI, betaII and delta) in the 3SBH5 cells. The amounts of PKC beta I in the cytosol of the 3SBH5 cells decreased in the cells pretreated with PMA. Irradiation did not change the decrease of PKC beta I. In contrast, treatment with PMA had no effect on the distribution of the other PKC subtypes. PMA appears to influence processes of radiation-induced apoptosis through the change in the distribution of PKC beta I. In addition, it was demonstrated that immunoprecipitates by anti-PKC alpha antibody included Raf-1, one of stress response proteins, in 3SBH5 cells after irradiation. These results suggest that PKC alpha might participate as a regulator in radiation-induced apoptsis, but PKC beta I might influence the apoptosis indirectly through the PMA-induced change of the distribution. | |||||
| 書誌情報 |
Radiation and Homeostasis : Proceedings of the International Symposium of Radiation and Homeostasis, held in Kyoto, Japan, 13-16 July 2001(International Congress Series ; no. 1236) p. 299-301, 発行日 2002 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
