{"created":"2023-05-15T14:38:24.371361+00:00","id":49572,"links":{},"metadata":{"_buckets":{"deposit":"3ae411cb-0bc3-44bf-9a0e-5f5adba9dcac"},"_deposit":{"created_by":1,"id":"49572","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49572"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049572","sets":["1"]},"author_link":["776650","776654","776658","776661","776649","776660","776656","776652","776651","776659","776653","776655","776657"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"19","bibliographicPageEnd":"14861","bibliographicPageStart":"14849","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"Oncotarget"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Malignant mesothelioma (MM) is extremely aggressive and a typical refractory cancer. In this study we investigated how effective on killing MM cells by carbon ion beam alone or in combination with cisplatin (CDDP) . Carbon ion beam (at the center of SOBP with 50 keV/µm of average LET) dose-independently suppressed MM cells MESO-1 and H226 cell viability and in combination with CDDP (25 μM) significantly enhanced its action. Relative biological effectiveness (RBE) values at 73 keV/μm and 13 keV/μm portion of carbon ion beam was estimated as 2.82-2.93 and 1.19-1.22 at D10 level relative to X-ray, respectively by using colony formation assay. Quantitative real time PCR analysis showed that expression of apoptosis-related BAX and autophagy-related Beclin1 and ATG7 was significantly enhanced by carbon ion beam alone or in combination with CDDP. Apoptosis analysis showed that caspase 3/7 activity and the percentage of apoptotic cells was dose-dependently increased after carbon ion beam and it was further increased when combined with CDDP. Spheroid formation ability of cancer stem like CD44+/CD26+ cells was significantly inhibited by carbon ion beam combined with CDDP. Besides, carbon ion beam combined with cisplatin significantly inhibited cell cycle progression (sub-G1 arrest) and induced more large number of γH2AX foci. In conclusion, carbon ion beam combined with CDDP has superior potential to kill MM cells including CSCs with enhanced apoptosis.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Impact Journals"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"29599911","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.18632/oncotarget.23756","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1949-2553","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"776649","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Masao"}],"nameIdentifiers":[{"nameIdentifier":"776650","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ho Kim, Eun"}],"nameIdentifiers":[{"nameIdentifier":"776651","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hayashi, Mitsuhiro"}],"nameIdentifiers":[{"nameIdentifier":"776652","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Vares, Guillaume"}],"nameIdentifiers":[{"nameIdentifier":"776653","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamamoto, Naoyoshi"}],"nameIdentifiers":[{"nameIdentifier":"776654","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyamoto, Tadaaki"}],"nameIdentifiers":[{"nameIdentifier":"776655","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sai, Sei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"776656","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Masao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"776657","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ho Kim, Eun","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"776658","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Vares, Guillaume","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"776659","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamamoto, Naoyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"776660","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyamoto, Tadaaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"776661","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effects of carbon ion beam alone or in combination with cisplatin on malignant mesothelioma cells in vitro","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of carbon ion beam alone or in combination with cisplatin on malignant mesothelioma cells in vitro"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-02-07"},"publish_date":"2019-02-07","publish_status":"0","recid":"49572","relation_version_is_last":true,"title":["Effects of carbon ion beam alone or in combination with cisplatin on malignant mesothelioma cells in vitro"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:19:46.224957+00:00"}