{"created":"2023-05-15T14:38:24.152000+00:00","id":49567,"links":{},"metadata":{"_buckets":{"deposit":"03e73993-3fc2-44df-8a91-9bfc3f4ccc29"},"_deposit":{"created_by":1,"id":"49567","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49567"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049567","sets":["1"]},"author_link":["754679","754674","754678","754676","754677","754675"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"205","bibliographicPageStart":"196","bibliographicVolumeNumber":"205","bibliographic_titles":[{"bibliographic_title":"Journal of Structural Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Small-angle X-ray scattering experiments were carried out to investigate the structural changes of cardiac thin filaments induced by the cardiomyopathy-causing E244D mutation in troponin T (TnT). We examined native thin filaments (NTF) from a bovine heart, reconstituted thin filaments containing human cardiac wild-type Tn (WTF), and filaments containing the E244D mutant of Tn (DTF), in the absence and presence of Ca2+. Analysis by model calculation showed that upon Ca2+-activation, tropomyosin (Tm) and Tn in the WTF and NTF moved together in a direction to expose myosin-binding sites on actin. On the other hand, Tm and Tn of the DTF moved in the opposite directions to each other upon Ca2+-activation. These movements caused Tm to expose more myosin-binding sites on actin than the WTF, suggesting that the affinity of myosin for actin is higher for the DTF. Thus, the mutation-induced structural changes in thin filaments would increase the number of myosin molecules bound to actin compared with the WTF, resulting in the force enhancement observed for the E244D mutation.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"30599212","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jsb.2018.12.005","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/pii/S1047847718303186","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1047-8477","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"松尾, 龍人"}],"nameIdentifiers":[{"nameIdentifier":"754674","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kono, Fumiaki"}],"nameIdentifiers":[{"nameIdentifier":"754675","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤原, 悟"}],"nameIdentifiers":[{"nameIdentifier":"754676","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsuo, Tatsuhito","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"754677","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kono, Fumiaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"754678","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujiwara, Satoru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"754679","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effects of the cardiomyopathy-causing E244D mutation of troponin T on the structures of cardiac thin filaments studied by small-angle X-ray scattering","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of the cardiomyopathy-causing E244D mutation of troponin T on the structures of cardiac thin filaments studied by small-angle X-ray scattering"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-02-06"},"publish_date":"2019-02-06","publish_status":"0","recid":"49567","relation_version_is_last":true,"title":["Effects of the cardiomyopathy-causing E244D mutation of troponin T on the structures of cardiac thin filaments studied by small-angle X-ray scattering"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:50:18.040679+00:00"}