{"created":"2023-05-15T14:38:21.908567+00:00","id":49515,"links":{},"metadata":{"_buckets":{"deposit":"ae8e5902-76a7-4ee0-899a-6b34e34355c8"},"_deposit":{"created_by":1,"id":"49515","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49515"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049515","sets":["1"]},"author_link":["500120","500119","500116","500114","500122","500117","500115","500118","500121"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"Int. J. Microgravity Sci. Appl."}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Human MTH1 hydrolyzes oxidized nucleoside triphosphates with broad substrate specificity and draws attention as a potential anticancer\ntarget. Recently, we determined the high resolution crystal structures of MTH1 and suggested that MTH1 recognizes different substrates via an\nexchange of the protonation state at Asp119 and Asp120. In order to validate this mechanism, it is essential to observe hydrogen atoms by\nultra-high resolution X-ray crystallography and/or neutron crystallography using large high quality crystals. Here we carried out the\ncrystallization of MTH1 in complex with a substrate, 8-oxo-dGTP, under microgravity in the Japanese Experiment Module ‘Kibo’. One of the\ncrystals diffracted to 1.04-Å resolution, which is better than that we reported previously. We carried out bond length analysis of Asp119 and\nAsp120 using this updated data, which revealed the protonation state based on the bond lengths with higher accuracy and precision.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.15011//jasma.36.360103","subitem_relation_type_select":"DOI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中村, 照也"}],"nameIdentifiers":[{"nameIdentifier":"500114","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平田, 啓介"}],"nameIdentifiers":[{"nameIdentifier":"500115","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤宮, 佳奈"}],"nameIdentifiers":[{"nameIdentifier":"500116","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"池鯉鮒, 麻美"}],"nameIdentifiers":[{"nameIdentifier":"500117","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"有森, 貴夫"}],"nameIdentifiers":[{"nameIdentifier":"500118","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"玉田, 太郎"}],"nameIdentifiers":[{"nameIdentifier":"500119","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"池水, 信二"}],"nameIdentifiers":[{"nameIdentifier":"500120","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山縣, ゆり子"}],"nameIdentifiers":[{"nameIdentifier":"500121","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"玉田 太郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"500122","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"X-ray Structure Analysis of Human Oxidized Nucleotide Hydrolase MTH1 using Crystals Obtained under Microgravity","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"X-ray Structure Analysis of Human Oxidized Nucleotide Hydrolase MTH1 using Crystals Obtained under Microgravity"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-01-28"},"publish_date":"2019-01-28","publish_status":"0","recid":"49515","relation_version_is_last":true,"title":["X-ray Structure Analysis of Human Oxidized Nucleotide Hydrolase MTH1 using Crystals Obtained under Microgravity"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:19:21.299497+00:00"}