@article{oai:repo.qst.go.jp:00049509,
 author = {Sasaki, Nobuhiro and Watanabe, Aiko and Asakawa, Tomonori and Sasaki, Masato and Hoshi, Nobue and Naito, Zenbi and Furusawa, Yoshiya and Shimokawa, Takashi and Nishihara, Masahiro and Sasaki, Nobuhiro and Furusawa, Yoshiya and Shimokawa, Takashi and Nishihara, Masahiro},
 issue = {3},
 journal = {Plant Biotechnology},
 month = {Aug},
 note = {To develop new varieties of perennial plants, it generally needs a lengthy and laborious procedure. Here, we attempted to use ion beam irradiation mutagenesis that is considered one of the laborsaving tools to promote breeding of perennial plants. We evaluated the biological effect of five ion beam sources including C, Ne, Ar, Si and Fe and neutron on Japanese gentian and apple using the National Institute of Radiological Sciences (NIRS)-Heavy Ion Medical accelerator in Chiba (HIMAC) and Neutron exposure Accelerator System for Biological Effect experiments (NASBEE). As the results, the biological effects were observed by less than 10 Gy irradiation of ion beams in in vitro cultured gentian plants, whereas apple trees were less sensitive to ion beam irradiation after grafting. Growth of in vitro cultured gentians was repressed by 3 Gy of neutron irradiation, while that of grafted apple tree was not affected by 4 Gy irradiation. During in vitro proliferation, seven pink-flowered lines from original blue flower were obtained in gentian by C and Ne ion beam irradiation, in total. Genomic and RT-PCR analyses of these lines revealed that the mutations were definitely caused by the deletion of flavonoid 3’,5’-hydroxylase (F3’5’H) genomic region. These results provide useful information for the mutagenesis and breeding of gentian and apple tree in the future.},
 pages = {249--257},
 title = {Biological effect of ion beam irradiation on perennial gentian and apple},
 volume = {35},
 year = {2018}
}